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| | ==ubiquitin-like domain-containing C-terminal domain phosphatase (UBLCP1)== | | ==ubiquitin-like domain-containing C-terminal domain phosphatase (UBLCP1)== |
| - | <StructureSection load='2m17' size='340' side='right'caption='[[2m17]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2m17' size='340' side='right'caption='[[2m17]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2m17]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M17 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2m17]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M17 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UBLCP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m17 OCA], [https://pdbe.org/2m17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m17 RCSB], [https://www.ebi.ac.uk/pdbsum/2m17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m17 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m17 OCA], [https://pdbe.org/2m17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m17 RCSB], [https://www.ebi.ac.uk/pdbsum/2m17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m17 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/UBCP1_HUMAN UBCP1_HUMAN]] Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.<ref>PMID:15883030</ref> <ref>PMID:21949367</ref>
| + | [https://www.uniprot.org/uniprot/UBCP1_HUMAN UBCP1_HUMAN] Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.<ref>PMID:15883030</ref> <ref>PMID:21949367</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phosphoprotein phosphatase]]
| + | [[Category: Lee W]] |
| - | [[Category: Lee, W]] | + | [[Category: Yun J]] |
| - | [[Category: Yun, J]] | + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
UBCP1_HUMAN Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.[1] [2]
Publication Abstract from PubMed
The ubiquitin-like modifier (UBL) domain of ubiquitin-like domain proteins (UDPs) interacts specifically with subunits of the 26 S proteasome. A novel UDP, ubiquitin-like domain-containing C-terminal domain phosphatase (UBLCP1), has been identified as an interacting partner of the 26 S proteasome. We determined the high-resolution solution structure of the UBL domain of human UBLCP1 by nuclear magnetic resonance spectroscopy. The UBL domain of hUBLCP1 has a unique beta-strand (beta3) and beta3-alpha2 loop, instead of the canonical beta4 observed in other UBL domains. The molecular topology and secondary structures are different from those of known UBL domains including that of fly UBLCP1. Data from backbone dynamics shows that the beta3-alpha2 loop is relatively rigid although it might have intrinsic dynamic profile. The positively charged residues of the beta3-alpha2 loop are involved in interacting with the C-terminal leucine-rich repeat-like domain of Rpn1.
Solution Structure and Rpn1 Interaction of the UBL Domain of Human RNA Polymerase II C-Terminal Domain Phosphatase.,Yun JH, Ko S, Lee CK, Cheong HK, Cheong C, Yoon JB, Lee W PLoS One. 2013 May 7;8(5):e62981. doi: 10.1371/journal.pone.0062981. Print 2013. PMID:23667555[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zheng H, Ji C, Gu S, Shi B, Wang J, Xie Y, Mao Y. Cloning and characterization of a novel RNA polymerase II C-terminal domain phosphatase. Biochem Biophys Res Commun. 2005 Jun 17;331(4):1401-7. PMID:15883030 doi:S0006-291X(05)00831-4
- ↑ Guo X, Engel JL, Xiao J, Tagliabracci VS, Wang X, Huang L, Dixon JE. UBLCP1 is a 26S proteasome phosphatase that regulates nuclear proteasome activity. Proc Natl Acad Sci U S A. 2011 Sep 26. PMID:21949367 doi:10.1073/pnas.1113170108
- ↑ Yun JH, Ko S, Lee CK, Cheong HK, Cheong C, Yoon JB, Lee W. Solution Structure and Rpn1 Interaction of the UBL Domain of Human RNA Polymerase II C-Terminal Domain Phosphatase. PLoS One. 2013 May 7;8(5):e62981. doi: 10.1371/journal.pone.0062981. Print 2013. PMID:23667555 doi:10.1371/journal.pone.0062981
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