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4qn0

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Revision as of 13:40, 22 February 2023

Crystal structure of the CPS-6 mutant Q130K

Structural highlights

4qn0 is a 4 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NUCG_CAEEL Endonuclease important for programmed cell death; it mediates apoptotic DNA fragmentation.

Publication Abstract from PubMed

Endonuclease G (EndoG) is a mitochondrial protein that is released from mitochondria and relocated into the nucleus to promote chromosomal DNA fragmentation during apoptosis. Here, we show that oxidative stress causes cell-death defects in C. elegans through an EndoG-mediated cell-death pathway. In response to high reactive oxygen species (ROS) levels, homodimeric CPS-6-the C. elegans homolog of EndoG-is dissociated into monomers with diminished nuclease activity. Conversely, the nuclease activity of CPS-6 is enhanced, and its dimeric structure is stabilized by its interaction with the worm AIF homolog, WAH-1, which shifts to disulfide cross-linked dimers under high ROS levels. CPS-6 thus acts as a ROS sensor to regulate the life and death of cells. Modulation of the EndoG dimer conformation could present an avenue for prevention and treatment of diseases resulting from oxidative stress.

Oxidative Stress Impairs Cell Death by Repressing the Nuclease Activity of Mitochondrial Endonuclease G.,Lin JL, Nakagawa A, Skeen-Gaar R, Yang WZ, Zhao P, Zhang Z, Ge X, Mitani S, Xue D, Yuan HS Cell Rep. 2016 Jul 12;16(2):279-87. doi: 10.1016/j.celrep.2016.05.090. Epub 2016 , Jun 23. PMID:27346342^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lin JL, Nakagawa A, Skeen-Gaar R, Yang WZ, Zhao P, Zhang Z, Ge X, Mitani S, Xue D, Yuan HS. Oxidative Stress Impairs Cell Death by Repressing the Nuclease Activity of Mitochondrial Endonuclease G. Cell Rep. 2016 Jul 12;16(2):279-87. doi: 10.1016/j.celrep.2016.05.090. Epub 2016 , Jun 23. PMID:27346342 doi:http://dx.doi.org/10.1016/j.celrep.2016.05.090

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4qn0"

Categories: Caenorhabditis elegans | Large Structures | Lin JLJ | Yuan HS

@@ Line 3: / Line 3: @@
 <StructureSection load='4qn0' size='340' side='right'caption='[[4qn0]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4qn0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QN0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4qn0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QN0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3s5b|3s5b]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qn0 OCA], [https://pdbe.org/4qn0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qn0 RCSB], [https://www.ebi.ac.uk/pdbsum/4qn0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qn0 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cps-6, C41D11.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qn0 OCA], [http://pdbe.org/4qn0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qn0 RCSB], [http://www.ebi.ac.uk/pdbsum/4qn0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qn0 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NUCG_CAEEL NUCG_CAEEL]] Endonuclease important for programmed cell death; it mediates apoptotic DNA fragmentation.
+[https://www.uniprot.org/uniprot/NUCG_CAEEL NUCG_CAEEL] Endonuclease important for programmed cell death; it mediates apoptotic DNA fragmentation.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 20: @@
 ==See Also==
-*[[Endonuclease|Endonuclease]]
+*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Caeel]]
+[[Category: Caenorhabditis elegans]]
 [[Category: Large Structures]]
-[[Category: Lin, J L.J]]
+[[Category: Lin JLJ]]
-[[Category: Yuan, H S]]
+[[Category: Yuan HS]]
-[[Category: Beta-beta-alpha metal motif]]
-[[Category: Endoribonuclease]]
-[[Category: Hydrolase]]
-[[Category: Mitochondrial membrane]]

4qn0

From Proteopedia

Revision as of 13:40, 22 February 2023

Crystal structure of the CPS-6 mutant Q130K

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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