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| | ==Structure of human NAPE-PLD== | | ==Structure of human NAPE-PLD== |
| - | <StructureSection load='4qn9' size='340' side='right' caption='[[4qn9]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='4qn9' size='340' side='right'caption='[[4qn9]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4qn9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QN9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QN9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4qn9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QN9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=DXC:(3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC+ACID'>DXC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=DXC:(3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC+ACID'>DXC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1m5t|1m5t]], [[4do3|4do3]], [[3zwf|3zwf]], [[1y44|1y44]], [[1bmc|1bmc]], [[1byr|1byr]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qn9 OCA], [https://pdbe.org/4qn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qn9 RCSB], [https://www.ebi.ac.uk/pdbsum/4qn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qn9 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C7orf18, NAPEPLD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylphosphatidylethanolamine-hydrolyzing_phospholipase_D N-acetylphosphatidylethanolamine-hydrolyzing phospholipase D], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.54 3.1.4.54] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qn9 OCA], [http://pdbe.org/4qn9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qn9 RCSB], [http://www.ebi.ac.uk/pdbsum/4qn9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qn9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NAPEP_HUMAN NAPEP_HUMAN]] Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors (By similarity). | + | [https://www.uniprot.org/uniprot/NAPEP_HUMAN NAPEP_HUMAN] Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: N-acetylphosphatidylethanolamine-hydrolyzing phospholipase D]] | + | [[Category: Large Structures]] |
| - | [[Category: Garau, G]] | + | [[Category: Garau G]] |
| - | [[Category: Acyl]]
| + | |
| - | [[Category: Aea]]
| + | |
| - | [[Category: Alpha-beta-beta-alpha fold]]
| + | |
| - | [[Category: Anandamide]]
| + | |
| - | [[Category: Bile acid]]
| + | |
| - | [[Category: Cannabinoid]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Deoxycholate]]
| + | |
| - | [[Category: Drug]]
| + | |
| - | [[Category: Fat]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Inflammation]]
| + | |
| - | [[Category: Mbl]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Nae]]
| + | |
| - | [[Category: Nape]]
| + | |
| - | [[Category: Obesity]]
| + | |
| - | [[Category: Oea]]
| + | |
| - | [[Category: Pain]]
| + | |
| - | [[Category: Pe]]
| + | |
| - | [[Category: Pea]]
| + | |
| - | [[Category: Phosphodiesterase]]
| + | |
| - | [[Category: Phospholipase]]
| + | |
| - | [[Category: Phospholipid]]
| + | |
| - | [[Category: Pld]]
| + | |
| - | [[Category: Steroid]]
| + | |
| Structural highlights
Function
NAPEP_HUMAN Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors (By similarity).
Publication Abstract from PubMed
The fatty acid ethanolamides (FAEs) are lipid mediators present in all organisms and involved in highly conserved biological functions, such as innate immunity, energy balance, and stress control. They are produced from membrane N-acylphosphatidylethanolamines (NAPEs) and include agonists for G protein-coupled receptors (e.g., cannabinoid receptors) and nuclear receptors (e.g., PPAR-alpha). Here, we report the crystal structure of human NAPE-hydrolyzing phospholipase D (NAPE-PLD) at 2.65 A resolution, a membrane enzyme that catalyzes FAE formation in mammals. NAPE-PLD forms homodimers partly separated by an internal approximately 9-A-wide channel and uniquely adapted to associate with phospholipids. A hydrophobic cavity provides an entryway for NAPE into the active site, where a binuclear Zn(2+) center orchestrates its hydrolysis. Bile acids bind with high affinity to selective pockets in this cavity, enhancing dimer assembly and enabling catalysis. These elements offer multiple targets for the design of small-molecule NAPE-PLD modulators with potential applications in inflammation and metabolic disorders.
Structure of human N-acylphosphatidylethanolamine-hydrolyzing phospholipase D: regulation of fatty acid ethanolamide biosynthesis by bile acids.,Magotti P, Bauer I, Igarashi M, Babagoli M, Marotta R, Piomelli D, Garau G Structure. 2015 Mar 3;23(3):598-604. doi: 10.1016/j.str.2014.12.018. Epub 2015, Feb 12. PMID:25684574[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Magotti P, Bauer I, Igarashi M, Babagoli M, Marotta R, Piomelli D, Garau G. Structure of human N-acylphosphatidylethanolamine-hydrolyzing phospholipase D: regulation of fatty acid ethanolamide biosynthesis by bile acids. Structure. 2015 Mar 3;23(3):598-604. doi: 10.1016/j.str.2014.12.018. Epub 2015, Feb 12. PMID:25684574 doi:http://dx.doi.org/10.1016/j.str.2014.12.018
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