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| | <StructureSection load='4r0m' size='340' side='right'caption='[[4r0m]], [[Resolution|resolution]] 2.45Å' scene=''> | | <StructureSection load='4r0m' size='340' side='right'caption='[[4r0m]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4r0m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Microcystis_aeruginosa_pcc_7806 Microcystis aeruginosa pcc 7806]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R0M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4r0m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_aeruginosa_PCC_7806 Microcystis aeruginosa PCC 7806]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R0M FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [https://pdbe.org/4r0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [https://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mcyG, IPF_370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=267872 Microcystis aeruginosa PCC 7806])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [http://pdbe.org/4r0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [http://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A8YJW1_MICAE A8YJW1_MICAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Microcystis aeruginosa pcc 7806]] | + | [[Category: Microcystis aeruginosa PCC 7806]] |
| - | [[Category: Chen, Y X]] | + | [[Category: Chen YX]] |
| - | [[Category: Dai, Y N]] | + | [[Category: Dai YN]] |
| - | [[Category: Jiang, Y L]] | + | [[Category: Jiang YL]] |
| - | [[Category: Ren, Y M]] | + | [[Category: Ren YM]] |
| - | [[Category: Tan, X F]] | + | [[Category: Tan XF]] |
| - | [[Category: Zhou, C Z]] | + | [[Category: Zhou CZ]] |
| - | [[Category: Zhou, K]] | + | [[Category: Zhou K]] |
| - | [[Category: Acetyl-coa synthetase-like domain]]
| + | |
| - | [[Category: Acyl carrier protein-like domain]]
| + | |
| - | [[Category: Adenylation domain]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Peptidyl carrier protein like domain]]
| + | |
| - | [[Category: Phenylalanyl-amp]]
| + | |
| - | [[Category: Phenylalanyl-amp binding]]
| + | |
| Structural highlights
Function
A8YJW1_MICAE
Publication Abstract from PubMed
Microcystins, which are the most common cause of hepatotoxicity associated with cyanobacterial water blooms, are assembled in vivo on a large multienzyme complex via a mixed nonribosomal peptide synthetase/polyketide synthetase (NRPS/PKS). The biosynthesis of microcystin in Microcystis aeruginosa PCC 7806 starts with the enzyme McyG, which contains an adenylation-peptidyl carrier protein (A-PCP) didomain for loading the starter unit to assemble the side chain of an Adda residue. However, the catalytic mechanism remains unclear. Here, the 2.45 A resolution crystal structure of the McyG A-PCP didomain complexed with the catalytic intermediate L-phenylalanyl-adenylate (L-Phe-AMP) is reported. Each asymmetric unit contains two protein molecules, one of which consists of the A-PCP didomain and the other of which comprises only the A domain. Structural analyses suggest that Val227 is likely to be critical for the selection of hydrophobic substrates. Moreover, two distinct interfaces demonstrating variable crosstalk between the PCP domain and the A domain were observed. A catalytic cycle for the adenylation and peptide transfer of the A-PCP didomain is proposed.
Structure of the adenylation-peptidyl carrier protein didomain of the Microcystis aeruginosa microcystin synthetase McyG.,Tan XF, Dai YN, Zhou K, Jiang YL, Ren YM, Chen Y, Zhou CZ Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):873-81. doi:, 10.1107/S1399004715001716. Epub 2015 Mar 27. PMID:25849398[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tan XF, Dai YN, Zhou K, Jiang YL, Ren YM, Chen Y, Zhou CZ. Structure of the adenylation-peptidyl carrier protein didomain of the Microcystis aeruginosa microcystin synthetase McyG. Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):873-81. doi:, 10.1107/S1399004715001716. Epub 2015 Mar 27. PMID:25849398 doi:http://dx.doi.org/10.1107/S1399004715001716
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