1k29
From Proteopedia
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| - | + | {{STRUCTURE_1k29| PDB=1k29 | SCENE= }} | |
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'''Solution Structure of a DNA Duplex Containing M1G Opposite a 2 Base Pair Deletion''' | '''Solution Structure of a DNA Duplex Containing M1G Opposite a 2 Base Pair Deletion''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K29 OCA]. | |
==Reference== | ==Reference== | ||
The exocyclic 1,N2-deoxyguanosine pyrimidopurinone M1G is a chemically stable DNA adduct when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene., Schnetz-Boutaud NC, Saleh S, Marnett LJ, Stone MP, Biochemistry. 2001 Dec 25;40(51):15638-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11747439 11747439] | The exocyclic 1,N2-deoxyguanosine pyrimidopurinone M1G is a chemically stable DNA adduct when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene., Schnetz-Boutaud NC, Saleh S, Marnett LJ, Stone MP, Biochemistry. 2001 Dec 25;40(51):15638-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11747439 11747439] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Marnett, L J.]] | [[Category: Marnett, L J.]] | ||
[[Category: Saleh, S.]] | [[Category: Saleh, S.]] | ||
[[Category: Schnetz-Boutaud, N C.]] | [[Category: Schnetz-Boutaud, N C.]] | ||
[[Category: Stone, M P.]] | [[Category: Stone, M P.]] | ||
| - | [[Category: | + | [[Category: Dna adduct]] |
| - | [[Category: | + | [[Category: Malondialdehyde]] |
| - | [[Category: | + | [[Category: Two base deletion]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:12:40 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:12, 2 May 2008
Solution Structure of a DNA Duplex Containing M1G Opposite a 2 Base Pair Deletion
Overview
The pyrimidopurinone adduct M1G [3-(2'-deoxy-beta-D-erythro-pentofuranosyl)pyrimido[1,2-a]-purin-10(3H)-on e], formed in DNA upon exposure to malondialdehyde or base propenals, was incorporated into 5'-d(ATCGCMCGGCATG)-3'-5'-d(CATGCCGCGAT)-3', where M = M1G. This duplex contained a two-nucleotide bulge in the modified strand, and was named the M1G-2BD oligodeoxynucleotide. It provided a model for -2 bp strand slippage deletions associated with the (CpG)3-iterated repeat hotspot for frameshift mutations from the Salmonella typhimurium hisD3052 gene. M1G was chemically stable in the M1G-2BD duplex at neutral pH. The two-base bulge in the M1G-2BD oligodeoxynucleotide was localized and consisted of M1G and the 3'-neighbor deoxycytosine. The intrahelical orientation of M1G was established from a combination of NOE and chemical shift data. M1G was in the anti conformation about the glycosyl bond. The 3'-neighbor deoxycytosine appeared to be extruded toward the major groove. In contrast, when M1G was placed into the corresponding fully complementary (CpG)3-iterated repeat duplex at neutral pH, spontaneous and quantitative ring-opening to N(2)-(3-oxo-1-propenyl)-dG (the OPG adduct) was facilitated [Mao, H., Reddy, G. R., Marnett, L. J., and Stone, M. P. (1999) Biochemistry 38, 13491-13501]. The structure of the M1G-2BD duplex suggested that the bulged sequence lacked a cytosine amino group properly positioned to facilitate opening of M1G and supports the notion that proper positioning of deoxycytosine complementary to M1G is necessary to promote ring-opening of the exocyclic adduct in duplex DNA. The structure of the M1G-2BD duplex was similar to that of the structural analogue 1,N(2)-propanodeoxyguanosine (PdG) in the corresponding PdG-2BD duplex [Weisenseel, J. P., Moe, J. G., Reddy, G. R., Marnett, L. J., and Stone, M. P. (1995) Biochemistry 34, 50-64]. The fixed position of the bulged bases in both instances suggests that these exocyclic adducts do not facilitate transient bulge migration.
About this Structure
Full crystallographic information is available from OCA.
Reference
The exocyclic 1,N2-deoxyguanosine pyrimidopurinone M1G is a chemically stable DNA adduct when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene., Schnetz-Boutaud NC, Saleh S, Marnett LJ, Stone MP, Biochemistry. 2001 Dec 25;40(51):15638-49. PMID:11747439 Page seeded by OCA on Fri May 2 22:12:40 2008
