7v55
From Proteopedia
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==Crystal structure of phospholipase D from Pseudomonas aeruginosa PAO1 using in situ proteolysis== | ==Crystal structure of phospholipase D from Pseudomonas aeruginosa PAO1 using in situ proteolysis== | ||
| - | <StructureSection load='7v55' size='340' side='right'caption='[[7v55]]' scene=''> | + | <StructureSection load='7v55' size='340' side='right'caption='[[7v55]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V55 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7v55]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V55 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v55 OCA], [https://pdbe.org/7v55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v55 RCSB], [https://www.ebi.ac.uk/pdbsum/7v55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v55 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v55 OCA], [https://pdbe.org/7v55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v55 RCSB], [https://www.ebi.ac.uk/pdbsum/7v55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v55 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9HYC2_PSEAE Q9HYC2_PSEAE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PldA, a phospholipase D (PLD) effector, catalyzes hydrolysis of the phosphodiester bonds of glycerophospholipids-the main component of cell membranes-and assists the invasion of the opportunistic pathogen Pseudomonas aeruginosa. As a cognate immunity protein, PA3488 can inhibit the activity of PldA to avoid self-toxicity. However, the precise inhibitory mechanism remains elusive. We determine the crystal structures of full-length and truncated PldA and the cryogenic electron microscopy structure of the PldA-PA3488 complex. Structural analysis reveals that there are different intermediates of PldA between the "open" and "closed" states of the catalytic pocket, accompanied by significant conformational changes in the "lid" region and the peripheral helical domain. Through structure-based mutational analysis, we identify the key residues responsible for the enzymatic activity of PldA. Together, these data provide an insight into the molecular mechanisms of PldA invasion and its neutralization by PA3488, aiding future design of PLD-targeted inhibitors and drugs. | ||
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| + | Structural insights into PA3488-mediated inactivation of Pseudomonas aeruginosa PldA.,Yang X, Li Z, Zhao L, She Z, Gao Z, Sui SF, Dong Y, Li Y Nat Commun. 2022 Oct 10;13(1):5979. doi: 10.1038/s41467-022-33690-2. PMID:36216841<ref>PMID:36216841</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7v55" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: Li Z]] | [[Category: Li Z]] | ||
[[Category: Yang Y]] | [[Category: Yang Y]] | ||
Revision as of 06:12, 2 March 2023
Crystal structure of phospholipase D from Pseudomonas aeruginosa PAO1 using in situ proteolysis
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