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| | ==NMR structure of TGAM_1934== | | ==NMR structure of TGAM_1934== |
| - | <StructureSection load='2mcf' size='340' side='right'caption='[[2mcf]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='2mcf' size='340' side='right'caption='[[2mcf]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mcf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thegj Thegj]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MCF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mcf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_gammatolerans_EJ3 Thermococcus gammatolerans EJ3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MCF FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TGAM_1934 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=593117 THEGJ])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mcf OCA], [https://pdbe.org/2mcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mcf RCSB], [https://www.ebi.ac.uk/pdbsum/2mcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mcf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mcf OCA], [https://pdbe.org/2mcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mcf RCSB], [https://www.ebi.ac.uk/pdbsum/2mcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mcf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C5A217_THEGJ C5A217_THEGJ] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thegj]] | + | [[Category: Thermococcus gammatolerans EJ3]] |
| - | [[Category: Guillen, K Montet de]] | + | [[Category: Montet de Guillen K]] |
| - | [[Category: Roumestand, C]] | + | [[Category: Roumestand C]] |
| - | [[Category: Yang, Y]] | + | [[Category: Yang Y]] |
| - | [[Category: Tgam]]
| + | |
| - | [[Category: Unknown function]]
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| Structural highlights
Function
C5A217_THEGJ
Publication Abstract from PubMed
ORFans are hypothetical proteins lacking any significant sequence similarity with other proteins. Here, we highlighted by quantitative proteomics the TGAM_1934 ORFan from the hyperradioresistant Thermococcus gammatolerans archaeon as one of the most abundant hypothetical proteins. This protein has been selected as a priority target for structure determination on the basis of its abundance in three cellular conditions. Its solution structure has been determined using multidimensional heteronuclear NMR spectroscopy. TGAM_1934 displays an original fold, although sharing some similarities with the 3D structure of the bacterial ortholog of frataxin, CyaY, a protein conserved in bacteria and eukaryotes and involved in iron-sulfur cluster biogenesis. These results highlight the potential of structural proteomics in prioritizing ORFan targets for structure determination based on quantitative proteomics data. The proteomic data and structure coordinates have been deposited to the ProteomeXchange with identifier PXD000402 (http://proteomecentral.proteomexchange.org/dataset/PXD000402) and Protein Data Bank under the accession number 2mcf, respectively.
Prioritizing targets for structural biology through the lens of proteomics: the archaeal protein TGAM_1934 from Thermococcus gammatolerans.,Yang YS, Fernandez B, Lagorce A, Aloin V, De Guillen KM, Boyer JB, Dedieu A, Confalonieri F, Armengaud J, Roumestand C Proteomics. 2015 Jan;15(1):114-23. doi: 10.1002/pmic.201300535. PMID:25359407[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yang YS, Fernandez B, Lagorce A, Aloin V, De Guillen KM, Boyer JB, Dedieu A, Confalonieri F, Armengaud J, Roumestand C. Prioritizing targets for structural biology through the lens of proteomics: the archaeal protein TGAM_1934 from Thermococcus gammatolerans. Proteomics. 2015 Jan;15(1):114-23. doi: 10.1002/pmic.201300535. PMID:25359407 doi:http://dx.doi.org/10.1002/pmic.201300535
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