1k2i
From Proteopedia
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'''Crystal Structure of Gamma-Chymotrypsin in Complex with 7-Hydroxycoumarin''' | '''Crystal Structure of Gamma-Chymotrypsin in Complex with 7-Hydroxycoumarin''' | ||
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[[Category: Ng, K K.S.]] | [[Category: Ng, K K.S.]] | ||
[[Category: Ullah, N.]] | [[Category: Ullah, N.]] | ||
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Revision as of 19:13, 2 May 2008
Crystal Structure of Gamma-Chymotrypsin in Complex with 7-Hydroxycoumarin
Overview
The 1.8 A crystal structure of 7-hydroxycoumarin (7-HC) bound to chymotrypsin reveals that this inhibitor forms a planar cinnamate acyl-enzyme complex. The phenyl ring of the bound inhibitor forms numerous van der Waals contacts in the S1 pocket of the enzyme, with the p-hydroxyl group donating a hydrogen bond to the main-chain oxygen atom of Ser217, and the o-hydroxyl group forming a water-mediated hydrogen bond with the carbonyl oxygen of Val227. The structure of the acyl-enzyme complex suggests that the mechanism of inhibition of 7-HC involves nucleophilic attack by the Ser195 O(gamma) atom on the carbonyl carbon atom of the inhibitor, accompanied by the breaking of the 2-pyrone ring of the inhibitor, and leading to the formation of a cinnamate acyl-enzyme derivative via a tetrahedral transition state. Comparisons with structures of photoreversible cinnamates bound to chymotrypsin reveal that although 7-HC interacts with the enzyme in a similar fashion, the binding of 7-HC to chymotrypsin takes place in a productive conformation in contrast to the photoreversible cinnamates. In summary, the 7-HC-chymotrypsin complex provides basic insight into the inhibition of chymotrypsin by natural coumarins and provides a structural basis for the design of more potent mechanism-based inhibitors against a wide range of biologically important chymotrypsin-like enzymes.
About this Structure
1K2I is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Crystal structure of gamma-chymotrypsin in complex with 7-hydroxycoumarin., Ghani U, Ng KK, Atta-ur-Rahman, Choudhary MI, Ullah N, James MN, J Mol Biol. 2001 Nov 30;314(3):519-25. PMID:11846564 Page seeded by OCA on Fri May 2 22:13:18 2008
