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| | <StructureSection load='4r4e' size='340' side='right'caption='[[4r4e]], [[Resolution|resolution]] 2.57Å' scene=''> | | <StructureSection load='4r4e' size='340' side='right'caption='[[4r4e]], [[Resolution|resolution]] 2.57Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4r4e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R4E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4r4e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R4E FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">glnR, BSU17450 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r4e OCA], [https://pdbe.org/4r4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r4e RCSB], [https://www.ebi.ac.uk/pdbsum/4r4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r4e ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r4e OCA], [http://pdbe.org/4r4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r4e RCSB], [http://www.ebi.ac.uk/pdbsum/4r4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r4e ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GLNR_BACSU GLNR_BACSU]] Represses many genes including the glnRA operon and tnrA during growth with nitrogen excess.<ref>PMID:10231480</ref> <ref>PMID:1677938</ref> <ref>PMID:2573733</ref> | + | [https://www.uniprot.org/uniprot/GLNR_BACSU GLNR_BACSU] Represses many genes including the glnRA operon and tnrA during growth with nitrogen excess.<ref>PMID:10231480</ref> <ref>PMID:1677938</ref> <ref>PMID:2573733</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacsu]] | + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Schumacher, M A]] | + | [[Category: Synthetic construct]] |
| - | [[Category: B. subtili]] | + | [[Category: Schumacher MA]] |
| - | [[Category: Dna]]
| + | |
| - | [[Category: Gs-q]]
| + | |
| - | [[Category: New member]]
| + | |
| - | [[Category: Nitrogen homeostasis]]
| + | |
| - | [[Category: Nucleoid]]
| + | |
| - | [[Category: Tnra/glnr dna-binding family of winged-hth with c-terminal inducer/sensor domain]]
| + | |
| - | [[Category: Tnra/glnr family]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulator-dna complex]]
| + | |
| Structural highlights
Function
GLNR_BACSU Represses many genes including the glnRA operon and tnrA during growth with nitrogen excess.[1] [2] [3]
Publication Abstract from PubMed
All cells must sense and adapt to changing nutrient availability. However, detailed molecular mechanisms coordinating such regulatory pathways remain poorly understood. In Bacillus subtilis, nitrogen homeostasis is controlled by a unique circuitry composed of the regulator TnrA, which is deactivated by feedback-inhibited glutamine synthetase (GS) during nitrogen excess and stabilized by GlnK upon nitrogen depletion, and the repressor GlnR. Here we describe a complete molecular dissection of this network. TnrA and GlnR, the global nitrogen homeostatic transcription regulators, are revealed as founders of a new structural family of dimeric DNA-binding proteins with C-terminal, flexible, effector-binding sensors that modulate their dimerization. Remarkably, the TnrA sensor domains insert into GS intersubunit catalytic pores, destabilizing the TnrA dimer and causing an unprecedented GS dodecamer-to-tetradecamer conversion, which concomitantly deactivates GS. In contrast, each subunit of the GlnK trimer "templates" active TnrA dimers. Unlike TnrA, GlnR sensors mediate an autoinhibitory dimer-destabilizing interaction alleviated by GS, which acts as a GlnR chaperone. Thus, these studies unveil heretofore unseen mechanisms by which inducible sensor domains drive metabolic reprograming in the model Gram-positive bacterium B. subtilis.
Structures of regulatory machinery reveal novel molecular mechanisms controlling B. subtilis nitrogen homeostasis.,Schumacher MA, Chinnam NB, Cuthbert B, Tonthat NK, Whitfill T Genes Dev. 2015 Feb 15;29(4):451-64. doi: 10.1101/gad.254714.114. PMID:25691471[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fisher SH. Regulation of nitrogen metabolism in Bacillus subtilis: vive la difference! Mol Microbiol. 1999 Apr;32(2):223-32. PMID:10231480
- ↑ Nakano Y, Kimura K. Purification and characterization of a repressor for the Bacillus cereus glnRA operon. J Biochem. 1991 Feb;109(2):223-8. PMID:1677938
- ↑ Schreier HJ, Brown SW, Hirschi KD, Nomellini JF, Sonenshein AL. Regulation of Bacillus subtilis glutamine synthetase gene expression by the product of the glnR gene. J Mol Biol. 1989 Nov 5;210(1):51-63. PMID:2573733
- ↑ Schumacher MA, Chinnam NB, Cuthbert B, Tonthat NK, Whitfill T. Structures of regulatory machinery reveal novel molecular mechanisms controlling B. subtilis nitrogen homeostasis. Genes Dev. 2015 Feb 15;29(4):451-64. doi: 10.1101/gad.254714.114. PMID:25691471 doi:http://dx.doi.org/10.1101/gad.254714.114
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