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| | <StructureSection load='4re0' size='340' side='right'caption='[[4re0]], [[Resolution|resolution]] 2.35Å' scene=''> | | <StructureSection load='4re0' size='340' side='right'caption='[[4re0]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4re0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vulm7 Vulm7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RE0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RE0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4re0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vulcanisaeta_moutnovskia_768-28 Vulcanisaeta moutnovskia 768-28]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RE0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4re0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4re0 OCA], [https://pdbe.org/4re0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4re0 RCSB], [https://www.ebi.ac.uk/pdbsum/4re0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4re0 ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rdy|4rdy]], [[4rdz|4rdz]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VMUT_2255 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=985053 VULM7])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4re0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4re0 OCA], [http://pdbe.org/4re0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4re0 RCSB], [http://www.ebi.ac.uk/pdbsum/4re0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4re0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F0QXN6_VULM7 F0QXN6_VULM7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vulm7]] | + | [[Category: Vulcanisaeta moutnovskia 768-28]] |
| - | [[Category: Bzdrenga, J]] | + | [[Category: Bzdrenga J]] |
| - | [[Category: Chabriere, E]] | + | [[Category: Chabriere E]] |
| - | [[Category: Champion, C]] | + | [[Category: Champion C]] |
| - | [[Category: Elias, M]] | + | [[Category: Elias M]] |
| - | [[Category: Gonzalez, D]] | + | [[Category: Gonzalez D]] |
| - | [[Category: Gotthard, G]] | + | [[Category: Gotthard G]] |
| - | [[Category: Hiblot, J]] | + | [[Category: Hiblot J]] |
| - | [[Category: Aryldialkylesterase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Lactonase]]
| + | |
| - | [[Category: Parathion hydrolase]]
| + | |
| - | [[Category: Quorum sensing]]
| + | |
| Structural highlights
Function
F0QXN6_VULM7
Publication Abstract from PubMed
A new representative of the Phosphotriesterase-Like Lactonases (PLLs) family from the hyperthermophilic crenarchaeon Vulcanisaeta moutnovskia has been characterized and crystallized. VmoLac is a native, proficient lactonase with promiscuous, low phosphotriesterase activity. VmoLac therefore represents an interesting candidate for engineering studies, with the aim of developing an efficient bacterial quorum-quenching agent. Here, we provide an extensive biochemical and kinetic characterization of VmoLac and describe the X-ray structures of the enzyme bound to a fatty acid and to its cognate substrate 3-oxo-C10 AHL (Acyl-Homoserine Lactone). The structures highlight possible structural determinants that may be involved in its extreme thermal stability (Tm = 128 degrees C). Moreover, the structure reveals that the substrate binding mode of VmoLac significantly differs from those of its close homologues, possibly explaining the substrate specificity of the enzyme. Finally, we describe the specific interactions between the enzyme and its substrate, and discuss the possible lactone hydrolysis mechanism of VmoLac.
Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia.,Hiblot J, Bzdrenga J, Champion C, Chabriere E, Elias M Sci Rep. 2015 Feb 11;5:8372. doi: 10.1038/srep08372. PMID:25670483[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hiblot J, Bzdrenga J, Champion C, Chabriere E, Elias M. Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia. Sci Rep. 2015 Feb 11;5:8372. doi: 10.1038/srep08372. PMID:25670483 doi:http://dx.doi.org/10.1038/srep08372
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