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| | <StructureSection load='4rha' size='340' side='right'caption='[[4rha]], [[Resolution|resolution]] 1.75Å' scene=''> | | <StructureSection load='4rha' size='340' side='right'caption='[[4rha]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4rha]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salt1 Salt1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RHA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4rha]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._14028S Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RHA FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rha OCA], [https://pdbe.org/4rha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rha RCSB], [https://www.ebi.ac.uk/pdbsum/4rha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rha ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4erh|4erh]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ompA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=588858 SALT1])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rha OCA], [http://pdbe.org/4rha PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rha RCSB], [http://www.ebi.ac.uk/pdbsum/4rha PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rha ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H2UKZ3_SALT1 A0A0H2UKZ3_SALT1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Salt1]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]] |
| - | [[Category: Cuff, M E]] | + | [[Category: Cuff ME]] |
| - | [[Category: Edwards, A]] | + | [[Category: Edwards A]] |
| - | [[Category: Joachimiak, A]] | + | [[Category: Joachimiak A]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Savchenko A]] |
| - | [[Category: Savchenko, A]] | + | [[Category: Skarina T]] |
| - | [[Category: Skarina, T]] | + | |
| - | [[Category: Mcsg]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Ompa]]
| + | |
| - | [[Category: Outer membrane protein]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Putative peptidoglycan domain]]
| + | |
| Structural highlights
Function
A0A0H2UKZ3_SALT1
Publication Abstract from PubMed
Salmonella enterica serovar Typhimurium can induce both humoral and cell-mediated responses when establishing itself in the host. These responses are primarily stimulated against the lipopolysaccharide and major outer membrane (OM) proteins. OmpA is one of these major OM proteins. It comprises a N-terminal eight-stranded beta-barrel transmembrane domain and a C-terminal domain (OmpA(CTD) ). The OmpA(CTD) and its homologs are believed to bind to peptidoglycan (PG) within the periplasm, maintaining bacterial osmotic homeostasis and modulating the permeability and integrity of the OM. Here we present the first crystal structures of the OmpA(CTD) from two pathogens: S. typhimurium (STOmpA(CTD) ) in open and closed forms and causative agent of Lyme Disease Borrelia burgdorferi (BbOmpA(CTD) ), in closed form. In the open form of STOmpA(CTD) , an aspartate residue from a long beta2-alpha3 loop points into the binding pocket, suggesting that an anion group such as a carboxylate group from PG is favored at the binding site. In the closed form of STOmpA(CTD) and in the structure of BbOmpA(CTD) , a sulfate group from the crystallization buffer is tightly bound at the binding site. The differences between the closed and open forms of STOmpA(CTD) , suggest a large conformational change that includes an extension of alpha3 helix by ordering a part of beta2-alpha3 loop. We propose that the sulfate anion observed in these structures mimics the carboxylate group of PG when bound to STOmpA(CTD) suggesting PG-anchoring mechanism. In addition, the binding of PG or a ligand mimic may enhance dimerization of STOmpA(CTD) , or possibly that of full length STOmpA.
Insights into PG-binding, conformational change, and dimerization of the OmpA C-terminal domains from Salmonella enterica serovar Typhimurium and Borrelia burgdorferi.,Tan K, Deatherage Kaiser BL, Wu R, Cuff M, Fan Y, Bigelow L, Jedrzejczak RP, Adkins JN, Cort JR, Babnigg G, Joachimiak A Protein Sci. 2017 Sep;26(9):1738-1748. doi: 10.1002/pro.3209. Epub 2017 Jun 19. PMID:28580643[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tan K, Deatherage Kaiser BL, Wu R, Cuff M, Fan Y, Bigelow L, Jedrzejczak RP, Adkins JN, Cort JR, Babnigg G, Joachimiak A. Insights into PG-binding, conformational change, and dimerization of the OmpA C-terminal domains from Salmonella enterica serovar Typhimurium and Borrelia burgdorferi. Protein Sci. 2017 Sep;26(9):1738-1748. doi: 10.1002/pro.3209. Epub 2017 Jun 19. PMID:28580643 doi:http://dx.doi.org/10.1002/pro.3209
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