1k3i

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[[Image:1k3i.jpg|left|200px]]
[[Image:1k3i.jpg|left|200px]]
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{{Structure
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|PDB= 1k3i |SIZE=350|CAPTION= <scene name='initialview01'>1k3i</scene>, resolution 1.40&Aring;
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The line below this paragraph, containing "STRUCTURE_1k3i", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span>
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1k3i| PDB=1k3i | SCENE= }}
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|RELATEDENTRY=[[1gof|1GOF]], [[1gog|1GOG]], [[1goh|1GOH]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3i OCA], [http://www.ebi.ac.uk/pdbsum/1k3i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k3i RCSB]</span>
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'''Crystal Structure of the Precursor of Galactose Oxidase'''
'''Crystal Structure of the Precursor of Galactose Oxidase'''
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[[Category: Wilmot, C M.]]
[[Category: Wilmot, C M.]]
[[Category: 7 blade beta propeller]]
[[Category: 7 blade beta propeller]]
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[[Category: precursor of copper enzyme.]]
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[[Category: Precursor of copper enzyme.]]
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[[Category: prosequence form]]
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[[Category: Prosequence form]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:15:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:42:52 2008''
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Revision as of 19:15, 2 May 2008

Image:1k3i.jpg

Template:STRUCTURE 1k3i

Crystal Structure of the Precursor of Galactose Oxidase


Overview

Galactose oxidase (EC ) is a monomeric enzyme that contains a single copper ion and catalyses the stereospecific oxidation of primary alcohols to their corresponding aldehydes. The protein contains an unusual covalent thioether bond between a tyrosine, which acts as a radical center during the two-electron reaction, and a cysteine. The enzyme is produced in a precursor form lacking the thioether bond and also possessing an additional 17-aa pro-sequence at the N terminus. Previous work has shown that the aerobic addition of Cu(2+) to the precursor is sufficient to generate fully processed mature enzyme. The structure of the precursor protein has been determined to 1.4 A, revealing the location of the pro-sequence and identifying structural differences between the precursor and the mature protein. Structural alignment of the precursor and mature forms of galactose oxidase shows that five regions of main chain and some key residues of the active site differ significantly between the two forms. The precursor structure provides a starting point for modeling the chemistry of thioether bond formation and pro-sequence cleavage.

About this Structure

1K3I is a Single protein structure of sequence from Fusarium sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of the precursor of galactose oxidase: an unusual self-processing enzyme., Firbank SJ, Rogers MS, Wilmot CM, Dooley DM, Halcrow MA, Knowles PF, McPherson MJ, Phillips SE, Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):12932-7. PMID:11698678 Page seeded by OCA on Fri May 2 22:15:22 2008

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