7y7o
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of metallo-endoribonuclease YbeY from Staphylococcus aureus== | |
| + | <StructureSection load='7y7o' size='340' side='right'caption='[[7y7o]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7y7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y7O FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y7o OCA], [https://pdbe.org/7y7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y7o RCSB], [https://www.ebi.ac.uk/pdbsum/7y7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y7o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/YBEY_STAAM YBEY_STAAM] Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Endoribonuclease YbeY is specific to the single-stranded RNA of ribosomal RNAs and small RNAs. This enzyme is essential for the maturation and quality control of ribosomal RNA in a wide range of bacteria and for virulence in some pathogenic bacteria. In this study, we determined the crystal structure of YbeY from Staphylococcus aureus at a resolution of 1.9 A in the presence of zinc chloride. The structure showed a zinc ion at the active site and two molecules of tricarboxylic acid citrate, which were also derived from the crystallization conditions. Our structure showed the zinc ion-bound local environment at the molecular level for the first time. Molecular comparisons were performed between the carboxylic moieties of citrate and the phosphate moiety of the RNA backbone, and a model of YbeY in complex with a single strand of RNA was subsequently constructed. Our findings provide molecular insights into how the YbeY enzyme recognizes single-stranded RNA in bacteria. | ||
| - | + | Crystal Structure of the Metallo-Endoribonuclease YbeY from Staphylococcus aureus.,Lee J, Jo I, Kwon AR, Ha NC J Microbiol Biotechnol. 2023 Jan 28;33(1):28-34. doi: 10.4014/jmb.2209.09019. , Epub 2022 Nov 23. PMID:36457189<ref>PMID:36457189</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Ha | + | <div class="pdbe-citations 7y7o" style="background-color:#fffaf0;"></div> |
| - | [[Category: Lee | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Staphylococcus aureus subsp. aureus Mu50]] | ||
| + | [[Category: Ha N-C]] | ||
| + | [[Category: Lee J]] | ||
Revision as of 07:28, 8 March 2023
Crystal structure of metallo-endoribonuclease YbeY from Staphylococcus aureus
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