8c3f

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'''Unreleased structure'''
 
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The entry 8c3f is ON HOLD until Paper Publication
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==Double mutant I(L177)H/F(M197)H structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV==
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<StructureSection load='8c3f' size='340' side='right'caption='[[8c3f]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8c3f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides_2.4.1 Cereibacter sphaeroides 2.4.1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C3F FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c3f OCA], [https://pdbe.org/8c3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c3f RCSB], [https://www.ebi.ac.uk/pdbsum/8c3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c3f ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RCEH_CERSP RCEH_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The photosynthetic reaction center of the purple bacterium Cereibacter sphaeroides with two site-directed mutations Ile-L177-His and M197 Phe-His is of double interest. The substitution I(L177)H results in strong binding of a bacteriochlorophyll molecule with L-subunit. The second mutation F(M197)H introduces a new H-bond between the C2-acetyl carbonyl group of the bacteriochlorophyll P(B) and His-M197, which is known to enhance the stability of the complex. Due to this H-bond, pi -electron system of P finds itself connected to an extensive H-bonding network on the periplasmic surface of the complex. The crystal structure of the double mutant reaction center obtained with 2.6 A resolution allows clarifying consequences of the Ile L177 - His substitution. The value of the P/P(+) midpoint potential in the double mutant RC was found to be ~20 mV less than the sum of potentials measured in the two RCs with single mutations I(L177)H and F(M197)H. The protein environment of the BChls P(A) and B(B) were found to be similar to that in the RC with single substitution I(L177)H, whereas an altered pattern of the H-bonding networks was found in the vicinity of bacteriochlorophyll P(B). The data obtained are consistent with our previous assumption on a correlation between the bulk of the H-bonding network connected with the pi-electron system of the primary electron donor P and the value of its oxidation potential.
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Authors: Gabdulkhakov, A.G., Selikhanov, G.K., Fufina, T.Y., Vasilieva, L.G.
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Properties and Crystal Structure of the Cereibacter sphaeroides Photosynthetic Reaction Center with Double Amino Acid Substitution I(L177)H + F(M197)H.,Fufina TY, Selikhanov GK, Gabdulkhakov AG, Vasilieva LG Membranes (Basel). 2023 Jan 26;13(2):157. doi: 10.3390/membranes13020157. PMID:36837660<ref>PMID:36837660</ref>
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Description: Double mutant I(L177)H/F(M197)H structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Vasilieva, L.G]]
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<div class="pdbe-citations 8c3f" style="background-color:#fffaf0;"></div>
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[[Category: Gabdulkhakov, A.G]]
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== References ==
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[[Category: Selikhanov, G.K]]
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<references/>
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[[Category: Fufina, T.Y]]
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__TOC__
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</StructureSection>
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[[Category: Cereibacter sphaeroides 2 4.1]]
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[[Category: Large Structures]]
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[[Category: Fufina TY]]
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[[Category: Gabdulkhakov AG]]
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[[Category: Selikhanov GK]]
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[[Category: Vasilieva LG]]

Revision as of 07:34, 8 March 2023

Double mutant I(L177)H/F(M197)H structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV

PDB ID 8c3f

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