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| | ==Solution structure of the RNA recognition motif of human TAF15== | | ==Solution structure of the RNA recognition motif of human TAF15== |
| - | <StructureSection load='2mmy' size='340' side='right'caption='[[2mmy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mmy' size='340' side='right'caption='[[2mmy]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mmy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MMY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mmy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MMY FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TAF15 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mmy OCA], [https://pdbe.org/2mmy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mmy RCSB], [https://www.ebi.ac.uk/pdbsum/2mmy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mmy ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mmy OCA], [https://pdbe.org/2mmy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mmy RCSB], [https://www.ebi.ac.uk/pdbsum/2mmy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mmy ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[https://www.uniprot.org/uniprot/RBP56_HUMAN RBP56_HUMAN]] Extraskeletal myxoid chondrosarcoma. A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
| + | [https://www.uniprot.org/uniprot/RBP56_HUMAN RBP56_HUMAN] Extraskeletal myxoid chondrosarcoma. A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3. |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/RBP56_HUMAN RBP56_HUMAN]] RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).<ref>PMID:19124016</ref>
| + | [https://www.uniprot.org/uniprot/RBP56_HUMAN RBP56_HUMAN] RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).<ref>PMID:19124016</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bhavesh, N S]] | + | [[Category: Bhavesh NS]] |
| - | [[Category: Ganguly, A K]] | + | [[Category: Ganguly AK]] |
| - | [[Category: Kashyap, M]] | + | [[Category: Kashyap M]] |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rrm]]
| + | |
| - | [[Category: Taf15]]
| + | |
| Structural highlights
Disease
RBP56_HUMAN Extraskeletal myxoid chondrosarcoma. A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
Function
RBP56_HUMAN RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).[1]
Publication Abstract from PubMed
Human TATA binding protein associated factor 2 N (TAF15) and Fused in sarcoma (FUS) are nucleic acid binding proteins belonging to the conserved FET family of proteins. They are involved in diverse processes such as pre-mRNA splicing, mRNA transport, and DNA binding. The absence of information regarding the structural mechanism employed by the FET family in recognizing and discriminating their cognate and non-cognate RNA targets has hampered the attainment of consensus on modes of protein-RNA binding for this family. Our study provides a molecular basis of this RNA recognition using a combination of solution-state NMR spectroscopy, calorimetry, docking and molecular dynamics simulation. Analysis of TAF15-RRM solution structure and its binding with stem-loop RNA has yielded conclusive evidence of a non-canonical mode of RNA recognition. Rather than classical stacking interactions that occur across nitrogen bases and aromatic amino acids on ribonucleoprotein sites, moderate-affinity hydrogen bonding network between the nitrogen bases in the stem-loop RNA and a concave face on the RRM surface primarily mediate TAF15-RRM RNA interaction. We have compared the binding affinities across a set of single-stranded RNA oligonucleotides to conclusively establish that RNA binding is dependent upon structural elements in the RNA rather than sequence.
Structural delineation of stem-loop RNA binding by human TAF15 protein.,Kashyap M, Ganguly AK, Bhavesh NS Sci Rep. 2015 Nov 27;5:17298. doi: 10.1038/srep17298. PMID:26612539[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jobert L, Argentini M, Tora L. PRMT1 mediated methylation of TAF15 is required for its positive gene regulatory function. Exp Cell Res. 2009 Apr 15;315(7):1273-86. doi: 10.1016/j.yexcr.2008.12.008. Epub , 2008 Dec 24. PMID:19124016 doi:http://dx.doi.org/10.1016/j.yexcr.2008.12.008
- ↑ Kashyap M, Ganguly AK, Bhavesh NS. Structural delineation of stem-loop RNA binding by human TAF15 protein. Sci Rep. 2015 Nov 27;5:17298. doi: 10.1038/srep17298. PMID:26612539 doi:http://dx.doi.org/10.1038/srep17298
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