1k5f
From Proteopedia
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| - | + | {{STRUCTURE_1k5f| PDB=1k5f | SCENE= }} | |
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'''SOLUTION STRUCTURE OF THE S-STYRENE ADDUCT IN THE RAS61 SEQUENCE''' | '''SOLUTION STRUCTURE OF THE S-STYRENE ADDUCT IN THE RAS61 SEQUENCE''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5F OCA]. | |
==Reference== | ==Reference== | ||
The nonmutagenic (R)- and (S)-beta-(N(6)-adenyl)styrene oxide adducts are oriented in the major groove and show little perturbation to DNA structure., Hennard C, Finneman J, Harris CM, Harris TM, Stone MP, Biochemistry. 2001 Aug 21;40(33):9780-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11502171 11502171] | The nonmutagenic (R)- and (S)-beta-(N(6)-adenyl)styrene oxide adducts are oriented in the major groove and show little perturbation to DNA structure., Hennard C, Finneman J, Harris CM, Harris TM, Stone MP, Biochemistry. 2001 Aug 21;40(33):9780-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11502171 11502171] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Finneman, J.]] | [[Category: Finneman, J.]] | ||
[[Category: Harris, C M.]] | [[Category: Harris, C M.]] | ||
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[[Category: Hennard, C.]] | [[Category: Hennard, C.]] | ||
[[Category: Stone, M P.]] | [[Category: Stone, M P.]] | ||
| - | [[Category: | + | [[Category: Dna adduct]] |
| - | [[Category: | + | [[Category: Solution structure of dna]] |
| - | [[Category: | + | [[Category: Styrene]] |
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Revision as of 19:19, 2 May 2008
SOLUTION STRUCTURE OF THE S-STYRENE ADDUCT IN THE RAS61 SEQUENCE
Overview
Conformations of (R)-beta-(N(6)-adenyl)styrene oxide and (S)-beta-(N(6)-adenyl)styrene oxide adducts at position X(6) in d(CGGACXAGAAG).d(CTTCTTGTCCG), incorporating codons 60, 61 (underlined), and 62 of the human N-ras protooncogene, were refined from (1)H NMR data. These were designated as the beta-R(61,2) and beta-S(61,2) adducts. A total of 533 distance restraints and 162 dihedral restraints were used for the molecular dynamics calculations of the beta-S(61,2) adduct, while 518 distances and 163 dihedrals were used for the beta-R(61,2) adduct. The increased tether length of the beta-adducts results in two significant changes in adduct structure as compared to the corresponding alpha-styrenyl adducts [Stone, M. P., and Feng, B. (1996) Magn. Reson. Chem. 34, S105-S114]. First, it reduces the distortion introduced into the DNA duplex. For both the beta-R(61,2) and beta-S(61,2) adducts, the styrenyl moiety was positioned in the major groove of the duplex with little steric hindrance. Second, it mutes the influence of stereochemistry at the alpha-carbon such that both the beta-R(61,2) and beta-S(61,2) adducts exhibit similar conformations. The results were correlated with site-specific mutagenesis experiments that revealed the beta-R(61,2) and beta-S(61,2) adducts were not mutagenic and did not block polymerase bypass.
About this Structure
Full crystallographic information is available from OCA.
Reference
The nonmutagenic (R)- and (S)-beta-(N(6)-adenyl)styrene oxide adducts are oriented in the major groove and show little perturbation to DNA structure., Hennard C, Finneman J, Harris CM, Harris TM, Stone MP, Biochemistry. 2001 Aug 21;40(33):9780-91. PMID:11502171 Page seeded by OCA on Fri May 2 22:19:55 2008
