This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1j4w
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1j4w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j4w" /> '''COMPLEX OF THE KH3 and KH4 DOMAINS OF FBP W...)
Next diff →
Revision as of 15:30, 12 November 2007
|
COMPLEX OF THE KH3 and KH4 DOMAINS OF FBP WITH A SINGLE_STRANDED 29mer DNA OLIGONUCLEOTIDE FROM THE FUSE ELEMENT OF THE C-MYC ONCOGENE
Overview
Gene regulation can be tightly controlled by recognition of DNA, deformations that are induced by stress generated during transcription., The KH domains of the FUSE-binding protein (FBP), a regulator of c-myc, expression, bind in vivo and in vitro to the single-stranded far-upstream, element (FUSE), 1,500 base pairs upstream from the c-myc promoter. FBP, bound to FUSE acts through TFIIH at the promoter. Here we report the, solution structure of a complex between the KH3 and KH4 domains of FBP and, a 29-base single-stranded DNA from FUSE. The KH domains recognize two, sites, 9-10 bases in length, separated by 5 bases, with KH4 bound to the, 5' site and KH3 to the 3' site. The central portion of each site comprises, a tetrad of sequence 5'd-ATTC for KH4 and 5'd-TTTT for KH3. Dynamics, measurements show that the two KH domains bind as articulated modules to, single-stranded DNA, providing a flexible framework with which to, recognize transient, moving targets.
About this Structure
1J4W is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and dynamics of KH domains from FBP bound to single-stranded DNA., Braddock DT, Louis JM, Baber JL, Levens D, Clore GM, Nature. 2002 Feb 28;415(6875):1051-6. PMID:11875576
Page seeded by OCA on Mon Nov 12 17:37:22 2007
