This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
8i5a
From Proteopedia
(Difference between revisions)
m (Protected "8i5a" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==N-acetyl-(R)-beta-phenylalanine acylase, 2.75 angstrom resolution== | |
| + | <StructureSection load='8i5a' size='340' side='right'caption='[[8i5a]], [[Resolution|resolution]] 2.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8i5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_sp. Burkholderia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I5A FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i5a OCA], [https://pdbe.org/8i5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i5a RCSB], [https://www.ebi.ac.uk/pdbsum/8i5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i5a ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | N-Acetyl-(R)-beta-phenylalanine acylase is an enzyme that hydrolyzes the amide bond of N-acetyl-(R)-beta-phenylalanine to produce enantiopure (R)-beta-phenylalanine. In previous studies, Burkholderia sp. AJ110349 and Variovorax sp. AJ110348 were isolated as (R)-enantiomer-specific N-acetyl-(R)-beta-phenylalanine acylase-producing organisms and the properties of the native enzyme from Burkholderia sp. AJ110349 were characterized. In this study, structural analyses were carried out in order to investigate the structure-function relationships of the enzymes derived from both organisms. The recombinant N-acetyl-(R)-beta-phenylalanine acylases were crystallized by the hanging-drop vapor-diffusion method under multiple crystallization solution conditions. The crystals of the Burkholderia enzyme belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 112.70-112.97, c = 341.50-343.32 A, and were likely to contain two subunits in the asymmetric unit. The crystal structure was solved by the Se-SAD method, suggesting that two subunits in the asymmetric unit form a dimer. Each subunit was composed of three domains, and they showed structural similarity to the corresponding domains of the large subunit of N,N-dimethylformamidase from Paracoccus sp. strain DMF. The crystals of the Variovorax enzyme grew as twinned crystals and were not suitable for structure determination. Using size-exclusion chromatography with online static light-scattering analysis, the N-acetyl-(R)-beta-phenylalanine acylases were clarified to be dimeric in solution. | ||
| - | + | Expression, purification and crystallization of N-acetyl-(R)-beta-phenylalanine acylases derived from Burkholderia sp. AJ110349 and Variovorax sp. AJ110348 and structure determination of the Burkholderia enzyme.,Kato Y, Kawasaki H, Nakamatsu T, Matsuda N, Natsume R Acta Crystallogr F Struct Biol Commun. 2023 Mar 1;79(Pt 3):70-78. doi: , 10.1107/S2053230X23000730. Epub 2023 Feb 23. PMID:36862095<ref>PMID:36862095</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8i5a" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kato | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Burkholderia sp]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kato Y]] | ||
| + | [[Category: Natsume R]] | ||
Revision as of 09:54, 15 March 2023
N-acetyl-(R)-beta-phenylalanine acylase, 2.75 angstrom resolution
| |||||||||||
