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2n50

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Revision as of 10:19, 15 March 2023

Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis

Structural highlights

2n50 is a 1 chain structure with sequence from Enterococcus faecalis V583. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q82ZE9_ENTFA Carrier of the growing fatty acid chain in fatty acid biosynthesis.[RuleBase:RU003545]

Publication Abstract from PubMed

Enterococcus faecalis is a Gram-positive, commensal bacterium that lives in the gastrointestinal tracts of humans and other mammals. It causes severe infections because of high antibiotic resistance. E. faecalis can endure extremes of temperature and pH. Acyl carrier protein (ACP) is a key element in the biosynthesis of fatty acids responsible for acyl group shuttling and delivery. In this study, to understand the origin of high thermal stabilities of E. faecalis ACP (Ef-ACP), its solution structure was investigated for the first time. CD experiments showed that the melting temperature of Ef-ACP is 78.8 degrees C, which is much higher than that of Escherichia coli ACP (67.2 degrees C). The overall structure of Ef-ACP shows the common ACP folding pattern consisting of four alpha-helices (helix I (residues 3-17), helix II (residues 39-53), helix III (residues 60-64), and helix IV (residues 68-78)) connected by three loops. Unique Ef-ACP structural features include a hydrophobic interaction between Phe(45) in helix II and Phe(18) in the alpha1alpha2 loop and a hydrogen bonding between Ser(15) in helix I and Ile(20) in the alpha1alpha2 loop, resulting in its high thermal stability. Phe(45)-mediated hydrophobic packing may block acyl chain binding subpocket II entry. Furthermore, Ser(58) in the alpha2alpha3 loop in Ef-ACP, which usually constitutes a proline in other ACPs, exhibited slow conformational exchanges, resulting in the movement of the helix III outside the structure to accommodate a longer acyl chain in the acyl binding cavity. These results might provide insights into the development of antibiotics against pathogenic drug-resistant E. faecalis strains.

Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis.,Park YG, Jung MC, Song H, Jeong KW, Bang E, Hwang GS, Kim Y J Biol Chem. 2016 Jan 22;291(4):1692-702. doi: 10.1074/jbc.M115.674408. Epub 2015, Dec 2. PMID:26631734^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Park YG, Jung MC, Song H, Jeong KW, Bang E, Hwang GS, Kim Y. Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis. J Biol Chem. 2016 Jan 22;291(4):1692-702. doi: 10.1074/jbc.M115.674408. Epub 2015, Dec 2. PMID:26631734 doi:http://dx.doi.org/10.1074/jbc.M115.674408

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2n50"

@@ Line 1: / Line 1: @@
 ==Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis==
-<StructureSection load='2n50' size='340' side='right'caption='[[2n50]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2n50' size='340' side='right'caption='[[2n50]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2n50]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N50 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2n50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_V583 Enterococcus faecalis V583]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N50 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n4z|2n4z]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n50 OCA], [https://pdbe.org/2n50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n50 RCSB], [https://www.ebi.ac.uk/pdbsum/2n50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n50 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n50 OCA], [https://pdbe.org/2n50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n50 RCSB], [https://www.ebi.ac.uk/pdbsum/2n50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n50 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/Q82ZE9_ENTFA Q82ZE9_ENTFA]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[RuleBase:RU003545]
+[https://www.uniprot.org/uniprot/Q82ZE9_ENTFA Q82ZE9_ENTFA] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[RuleBase:RU003545]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Enterococcus faecalis V583]]
 [[Category: Large Structures]]
-[[Category: Jeong, K]]
+[[Category: Jeong K]]
-[[Category: Jung, M]]
+[[Category: Jung M]]
-[[Category: Kim, Y]]
+[[Category: Kim Y]]
-[[Category: Park, Y]]
+[[Category: Park Y]]
-[[Category: Song, H]]
+[[Category: Song H]]
-[[Category: Acyl carrier protein]]
-[[Category: Biosynthetic protein]]
-[[Category: Enterococcus faecali]]
-[[Category: Fatty acid synthase]]

2n50

From Proteopedia

Revision as of 10:19, 15 March 2023

Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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