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2n7a

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Revision as of 10:22, 15 March 2023

Solution structure of the human Siglec-8 lectin domain

Structural highlights

2n7a is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SIGL8_HUMAN Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Publication Abstract from PubMed

Siglec-8 is a human immune-inhibitory receptor that, when engaged by specific self-glycans, triggers eosinophil apoptosis and inhibits mast cell degranulation, providing an endogenous mechanism to down-regulate immune responses of these central inflammatory effector cells. Here we used solution NMR spectroscopy to dissect the fine specificity of Siglec-8 toward different sialylated and sulfated carbohydrate ligands and determined the structure of the Siglec-8 lectin domain in complex with its prime glycan target 6'-sulfo sialyl Lewis(x) A canonical motif for sialic acid recognition, extended by a secondary motif formed by unique loop regions, recognizing 6-O-sulfated galactose dictates tight specificity distinct from other Siglec family members and any other endogenous glycan recognition receptors. Structure-guided mutagenesis revealed key contacts of both interfaces to be equally essential for binding. Our work provides critical structural and mechanistic insights into how Siglec-8 selectively recognizes its glycan target, rationalizes the functional impact of site-specific glycan sulfation in modulating this lectin-glycan interaction, and will enable the rational design of Siglec-8-targeted agonists to treat eosinophil- and mast cell-related allergic and inflammatory diseases, such as asthma.

Structural basis for sulfation-dependent self-glycan recognition by the human immune-inhibitory receptor Siglec-8.,Propster JM, Yang F, Rabbani S, Ernst B, Allain FH, Schubert M Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):E4170-9. doi:, 10.1073/pnas.1602214113. Epub 2016 Jun 29. PMID:27357658^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Propster JM, Yang F, Rabbani S, Ernst B, Allain FH, Schubert M. Structural basis for sulfation-dependent self-glycan recognition by the human immune-inhibitory receptor Siglec-8. Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):E4170-9. doi:, 10.1073/pnas.1602214113. Epub 2016 Jun 29. PMID:27357658 doi:http://dx.doi.org/10.1073/pnas.1602214113

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2n7a"

@@ Line 1: / Line 1: @@
 ==Solution structure of the human Siglec-8 lectin domain==
-<StructureSection load='2n7a' size='340' side='right'caption='[[2n7a]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2n7a' size='340' side='right'caption='[[2n7a]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2n7a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N7A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2n7a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N7A FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n7b|2n7b]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n7a OCA], [https://pdbe.org/2n7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n7a RCSB], [https://www.ebi.ac.uk/pdbsum/2n7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n7a ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAF2, SIGLEC8 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n7a OCA], [https://pdbe.org/2n7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n7a RCSB], [https://www.ebi.ac.uk/pdbsum/2n7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n7a ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/SIGL8_HUMAN SIGL8_HUMAN]] Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
+[https://www.uniprot.org/uniprot/SIGL8_HUMAN SIGL8_HUMAN] Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Allain, F H.T]]
+[[Category: Allain FH-T]]
-[[Category: Ernst, B]]
+[[Category: Ernst B]]
-[[Category: Proepster, J M]]
+[[Category: Proepster JM]]
-[[Category: Rabbani, S]]
+[[Category: Rabbani S]]
-[[Category: Schubert, M]]
+[[Category: Schubert M]]
-[[Category: Yang, F]]
+[[Category: Yang F]]
-[[Category: Carbohydrate-binding receptor]]
-[[Category: I-type lectin]]
-[[Category: Saf-2]]
-[[Category: Sialic acid-binding immunoglobulin-like lectin 8]]
-[[Category: Siglec8]]
-[[Category: Structural protein]]

2n7a

From Proteopedia

Revision as of 10:22, 15 March 2023

Solution structure of the human Siglec-8 lectin domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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