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| | ==NMR structure of the prolactin receptor transmembrane domain== | | ==NMR structure of the prolactin receptor transmembrane domain== |
| - | <StructureSection load='2n7i' size='340' side='right'caption='[[2n7i]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2n7i' size='340' side='right'caption='[[2n7i]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n7i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N7I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n7i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N7I FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRLR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n7i OCA], [https://pdbe.org/2n7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n7i RCSB], [https://www.ebi.ac.uk/pdbsum/2n7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n7i ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n7i OCA], [https://pdbe.org/2n7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n7i RCSB], [https://www.ebi.ac.uk/pdbsum/2n7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n7i ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/PRLR_HUMAN PRLR_HUMAN]] This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.<ref>PMID:12580759</ref>
| + | [https://www.uniprot.org/uniprot/PRLR_HUMAN PRLR_HUMAN] This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.<ref>PMID:12580759</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bugge, K]] | + | [[Category: Bugge K]] |
| - | [[Category: Kragelund, B B]] | + | [[Category: Kragelund BB]] |
| - | [[Category: Hormone receptor]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Receptor]]
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| - | [[Category: Transmembrane domain]]
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| Structural highlights
Function
PRLR_HUMAN This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.[1]
Publication Abstract from PubMed
The prolactin receptor is an archetype member of the class I cytokine receptor family, comprising receptors with fundamental functions in biology as well as key drug targets. Structurally, each of these receptors represent an intriguing diversity, providing an exceptionally challenging target for structural biology. Here, we access the molecular architecture of the monomeric human prolactin receptor by combining experimental and computational efforts. We solve the NMR structure of its transmembrane domain in micelles and collect structural data on overlapping fragments of the receptor with small-angle X-ray scattering, native mass spectrometry and NMR spectroscopy. Along with previously published data, these are integrated by molecular modelling to generate a full receptor structure. The result provides the first full view of a class I cytokine receptor, exemplifying the architecture of more than 40 different receptor chains, and reveals that the extracellular domain is merely the tip of a molecular iceberg.
A combined computational and structural model of the full-length human prolactin receptor.,Bugge K, Papaleo E, Haxholm GW, Hopper JT, Robinson CV, Olsen JG, Lindorff-Larsen K, Kragelund BB Nat Commun. 2016 May 13;7:11578. doi: 10.1038/ncomms11578. PMID:27174498[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Trott JF, Hovey RC, Koduri S, Vonderhaar BK. Alternative splicing to exon 11 of human prolactin receptor gene results in multiple isoforms including a secreted prolactin-binding protein. J Mol Endocrinol. 2003 Feb;30(1):31-47. PMID:12580759
- ↑ Bugge K, Papaleo E, Haxholm GW, Hopper JT, Robinson CV, Olsen JG, Lindorff-Larsen K, Kragelund BB. A combined computational and structural model of the full-length human prolactin receptor. Nat Commun. 2016 May 13;7:11578. doi: 10.1038/ncomms11578. PMID:27174498 doi:http://dx.doi.org/10.1038/ncomms11578
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