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| | ==Crystal structure of yellow lupin LLPR-10.1A protein in complex with trans-zeatin== | | ==Crystal structure of yellow lupin LLPR-10.1A protein in complex with trans-zeatin== |
| - | <StructureSection load='4ryv' size='340' side='right' caption='[[4ryv]], [[Resolution|resolution]] 1.38Å' scene=''> | + | <StructureSection load='4ryv' size='340' side='right'caption='[[4ryv]], [[Resolution|resolution]] 1.38Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ryv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/European_yellow_lupine European yellow lupine]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RYV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ryv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lupinus_luteus Lupinus luteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RYV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZEA:(2E)-2-METHYL-4-(9H-PURIN-6-YLAMINO)BUT-2-EN-1-OL'>ZEA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZEA:(2E)-2-METHYL-4-(9H-PURIN-6-YLAMINO)BUT-2-EN-1-OL'>ZEA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bv1|1bv1]], [[1fm4|1fm4]], [[1icx|1icx]], [[1ifv|1ifv]], [[1xdf|1xdf]], [[2flh|2flh]], [[2qim|2qim]], [[3e85|3e85]], [[4q0k|4q0k]], [[4a80|4a80]], [[4a81|4a81]], [[3ie5|3ie5]], [[4a83|4a83]], [[4a85|4a85]], [[4a86|4a86]], [[4a87|4a87]], [[4a8u|4a8u]], [[4a8v|4a8v]], [[4gy9|4gy9]], [[4jhg|4jhg]], [[4psb|4psb]], [[4jhh|4jhh]], [[4jhi|4jhi]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ryv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryv OCA], [https://pdbe.org/4ryv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ryv RCSB], [https://www.ebi.ac.uk/pdbsum/4ryv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryv ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LLR18A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3873 European yellow lupine])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ryv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryv OCA], [http://pdbe.org/4ryv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ryv RCSB], [http://www.ebi.ac.uk/pdbsum/4ryv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/L18A_LUPLU L18A_LUPLU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: European yellow lupine]] | + | [[Category: Large Structures]] |
| - | [[Category: Bujacz, G]] | + | [[Category: Lupinus luteus]] |
| - | [[Category: Dolot, R]] | + | [[Category: Bujacz G]] |
| - | [[Category: Jaskolski, M]] | + | [[Category: Dolot R]] |
| - | [[Category: Michalska, K]] | + | [[Category: Jaskolski M]] |
| - | [[Category: Sikorski, M M]] | + | [[Category: Michalska K]] |
| - | [[Category: Sliwiak, J]] | + | [[Category: Sikorski MM]] |
| - | [[Category: Cytokinin]]
| + | [[Category: Sliwiak J]] |
| - | [[Category: Ligand binding]]
| + | |
| - | [[Category: Phytohormone binding protein]]
| + | |
| - | [[Category: Plant protein]]
| + | |
| - | [[Category: Pr-10 fold]]
| + | |
| - | [[Category: Trans-zeatin]]
| + | |
| Structural highlights
Function
L18A_LUPLU
Publication Abstract from PubMed
Plant pathogenesis-related class 10 (PR-10) proteins are a family of abundant proteins initially identified as elements of the plant defense system. The key structural feature suggesting PR-10 functionality is a huge hydrophobic cavity created in the protein interior by a scaffold composed of an extended beta-sheet wrapped around a long and flexible C-terminal alpha-helix. Several crystallographic and NMR studies have shown that the cavity can accommodate a variety of small molecule ligands, including phytohormones. The article describes approximately 1.3A resolution crystal structures of a Lupinus luteus PR-10 isoform LlPR-10.1A, in its free form and in complex with trans-zeatin, a naturally occurring plant hormone belonging to the cytokinin group. Moreover we present the structure of the same protein where the saturation with zeatin is not complete. This set of three crystal structures allows us to track the structural adaptation of the protein upon trans-zeatin docking, as well as the sequence of the ligand-binding events, step-by-step. In addition, titration of LlPR-10.1A with trans-zeatin monitored in solution by CD spectra, confirmed the pattern of structural adaptations deduced from the crystallographic studies. The ligand-biding mode shows no similarity to other zeatin complexes of PR-10 proteins. The present work, which describes the first atomic models of the same PR-10 protein with and without a physiological ligand, reveals that the conformation of LlPR-10.1A undergoes a significant structural rearrangement upon trans-zeatin binding.
Crystallographic and CD probing of ligand-induced conformational changes in a plant PR-10 protein.,Sliwiak J, Dolot R, Michalska K, Szpotkowski K, Bujacz G, Sikorski M, Jaskolski M J Struct Biol. 2015 Nov 28. pii: S1047-8477(15)30105-2. doi:, 10.1016/j.jsb.2015.11.008. PMID:26644353[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sliwiak J, Dolot R, Michalska K, Szpotkowski K, Bujacz G, Sikorski M, Jaskolski M. Crystallographic and CD probing of ligand-induced conformational changes in a plant PR-10 protein. J Struct Biol. 2015 Nov 28. pii: S1047-8477(15)30105-2. doi:, 10.1016/j.jsb.2015.11.008. PMID:26644353 doi:http://dx.doi.org/10.1016/j.jsb.2015.11.008
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