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| | <StructureSection load='4s2a' size='340' side='right'caption='[[4s2a]], [[Resolution|resolution]] 2.93Å' scene=''> | | <StructureSection load='4s2a' size='340' side='right'caption='[[4s2a]], [[Resolution|resolution]] 2.93Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4s2a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cauvc Cauvc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S2A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4s2a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides_CB15 Caulobacter vibrioides CB15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S2A FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4n7q|4n7q]], [[3epm|3epm]], [[3epn|3epn]], [[3epo|3epo]], [[4s25|4s25]], [[4s26|4s26]], [[4s27|4s27]], [[4s28|4s28]], [[4s29|4s29]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s2a OCA], [https://pdbe.org/4s2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s2a RCSB], [https://www.ebi.ac.uk/pdbsum/4s2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s2a ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">thiC, CC_2029 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190650 CAUVC])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphomethylpyrimidine_synthase Phosphomethylpyrimidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.17 4.1.99.17] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s2a OCA], [http://pdbe.org/4s2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s2a RCSB], [http://www.ebi.ac.uk/pdbsum/4s2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4s2a ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/THIC_CAUCR THIC_CAUCR]] Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.<ref>PMID:18953358</ref> | + | [https://www.uniprot.org/uniprot/THIC_CAUVC THIC_CAUVC] Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.<ref>PMID:18953358</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cauvc]] | + | [[Category: Caulobacter vibrioides CB15]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phosphomethylpyrimidine synthase]]
| + | [[Category: Abdelwahed S]] |
| - | [[Category: Abdelwahed, S]] | + | [[Category: Begley TP]] |
| - | [[Category: Begley, T P]] | + | [[Category: Ealick SE]] |
| - | [[Category: Ealick, S E]] | + | [[Category: Fenwick MK]] |
| - | [[Category: Fenwick, M K]] | + | [[Category: Mehta AP]] |
| - | [[Category: Mehta, A P]] | + | [[Category: Zhang Y]] |
| - | [[Category: Zhang, Y]] | + | |
| - | [[Category: Adomet and glutamate mutase]]
| + | |
| - | [[Category: Alpha-beta barrel]]
| + | |
| - | [[Category: Domain swapping]]
| + | |
| - | [[Category: Iron-sulfur cluster]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Radical sam superfamily]]
| + | |
| - | [[Category: Thiamin]]
| + | |
| - | [[Category: Vitamin b1]]
| + | |
| - | [[Category: Vitamin b12]]
| + | |
| Structural highlights
Function
THIC_CAUVC Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.[1]
Publication Abstract from PubMed
Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to generate a 5'-deoxyadenosyl radical. Canonical radical SAM enzymes are characterized by a beta-barrel-like fold and SAM anchors to the differentiated iron of the cluster, which is located near the amino terminus and within the beta-barrel, through its amino and carboxylate groups. Here we show that ThiC, the thiamin pyrimidine synthase in plants and bacteria, contains a tethered cluster-binding domain at its carboxy terminus that moves in and out of the active site during catalysis. In contrast to canonical radical SAM enzymes, we predict that SAM anchors to an additional active site metal through its amino and carboxylate groups. Superimposition of the catalytic domains of ThiC and glutamate mutase shows that these two enzymes share similar active site architectures, thus providing strong evidence for an evolutionary link between the radical SAM and adenosylcobalamin-dependent enzyme superfamilies.
Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase.,Fenwick MK, Mehta AP, Zhang Y, Abdelwahed SH, Begley TP, Ealick SE Nat Commun. 2015 Mar 27;6:6480. doi: 10.1038/ncomms7480. PMID:25813242[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chatterjee A, Li Y, Zhang Y, Grove TL, Lee M, Krebs C, Booker SJ, Begley TP, Ealick SE. Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily. Nat Chem Biol. 2008 Dec;4(12):758-65. Epub 2008 Oct 26. PMID:18953358 doi:10.1038/nchembio.121
- ↑ Fenwick MK, Mehta AP, Zhang Y, Abdelwahed SH, Begley TP, Ealick SE. Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase. Nat Commun. 2015 Mar 27;6:6480. doi: 10.1038/ncomms7480. PMID:25813242 doi:http://dx.doi.org/10.1038/ncomms7480
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