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| | <StructureSection load='4tkr' size='340' side='right'caption='[[4tkr]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='4tkr' size='340' side='right'caption='[[4tkr]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4tkr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TKR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TKR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4tkr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TKR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGL:B-2-OCTYLGLUCOSIDE'>BGL</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGL:B-2-OCTYLGLUCOSIDE'>BGL</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M637_10125, M638_09860, M640_06115, M641_06335, M643_01630, M644_08810, M645_06650 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 "Bacterium monocytogenes hominis" Nyfeldt 1932])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tkr OCA], [https://pdbe.org/4tkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tkr RCSB], [https://www.ebi.ac.uk/pdbsum/4tkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tkr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tkr OCA], [http://pdbe.org/4tkr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tkr RCSB], [http://www.ebi.ac.uk/pdbsum/4tkr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tkr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0B8R2U7_LISMN A0A0B8R2U7_LISMN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium monocytogenes hominis nyfeldt 1932]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Guo, Y]] | + | [[Category: Listeria monocytogenes]] |
| - | [[Category: Hendrickson, W A]] | + | [[Category: Guo Y]] |
| - | [[Category: Liu, Q]] | + | [[Category: Hendrickson WA]] |
| - | [[Category: NYCOMPS, New York Consortium on Membrane Protein Structure]] | + | [[Category: Liu Q]] |
| - | [[Category: Low energy]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Multiple crystal]]
| + | |
| - | [[Category: Native-sad phasing]]
| + | |
| - | [[Category: New york consortium on membrane protein structure]]
| + | |
| - | [[Category: Nycomp]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Transporter]]
| + | |
| Structural highlights
Function
A0A0B8R2U7_LISMN
Publication Abstract from PubMed
Anomalous diffraction signals from typical native macromolecules are very weak, frustrating their use in de novo structure determination. Here, native SAD procedures are described to enhance signal to noise in anomalous diffraction by using multiple crystals in combination with synchrotron X-rays at 6 keV. Increased anomalous signals were obtained at 6 keV compared with 7 keV X-ray energy, which was used for previous native SAD analyses. A feasibility test of multi-crystal-based native SAD phasing was performed at 3.2 A resolution for a known tyrosine protein kinase domain, and real-life applications were made to two novel membrane proteins at about 3.0 A resolution. The three applications collectively serve to validate the robust feasibility of native SAD phasing at lower energy.
Multi-crystal native SAD analysis at 6 keV.,Liu Q, Guo Y, Chang Y, Cai Z, Assur Z, Mancia F, Greene MI, Hendrickson WA Acta Crystallogr D Biol Crystallogr. 2014 Oct 1;70(Pt 10):2544-57. doi:, 10.1107/S1399004714013376. Epub 2014 Sep 30. PMID:25286840[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Liu Q, Guo Y, Chang Y, Cai Z, Assur Z, Mancia F, Greene MI, Hendrickson WA. Multi-crystal native SAD analysis at 6 keV. Acta Crystallogr D Biol Crystallogr. 2014 Oct 1;70(Pt 10):2544-57. doi:, 10.1107/S1399004714013376. Epub 2014 Sep 30. PMID:25286840 doi:http://dx.doi.org/10.1107/S1399004714013376
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