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| | <StructureSection load='4tn0' size='340' side='right'caption='[[4tn0]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='4tn0' size='340' side='right'caption='[[4tn0]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4tn0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_hb93-13 Campylobacter jejuni subsp. jejuni hb93-13]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TN0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4tn0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_HB93-13 Campylobacter jejuni subsp. jejuni HB93-13]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TN0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tn0 OCA], [https://pdbe.org/4tn0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tn0 RCSB], [https://www.ebi.ac.uk/pdbsum/4tn0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tn0 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CJJHB9313_0266 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360112 Campylobacter jejuni subsp. jejuni HB93-13])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tn0 OCA], [http://pdbe.org/4tn0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tn0 RCSB], [http://www.ebi.ac.uk/pdbsum/4tn0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tn0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Campylobacter jejuni subsp. jejuni hb93-13]] | + | [[Category: Campylobacter jejuni subsp. jejuni HB93-13]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boll, J M]] | + | [[Category: Boll JM]] |
| - | [[Category: Brown, D]] | + | [[Category: Brown D]] |
| - | [[Category: Fage, C D]] | + | [[Category: Fage CD]] |
| - | [[Category: Keatinge-Clay, A T]] | + | [[Category: Keatinge-Clay AT]] |
| - | [[Category: Trent, M S]] | + | [[Category: Trent MS]] |
| - | [[Category: Alkaline phosphatase-like]]
| + | |
| - | [[Category: Periplasm]]
| + | |
| - | [[Category: Phosphoethanolamine transferase]]
| + | |
| - | [[Category: Phosphothreonine]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The foodborne enteric pathogen Campylobacter jejuni decorates a variety of its cell-surface structures with phosphoethanolamine (pEtN). Modifying lipid A with pEtN promotes cationic antimicrobial peptide resistance, whereas post-translationally modifying the flagellar rod protein FlgG with pEtN promotes flagellar assembly and motility, which are processes that are important for intestinal colonization. EptC, the pEtN transferase required for all known pEtN cell-surface modifications in C. jejuni, is a predicted inner-membrane metalloenzyme with a five-helix N-terminal transmembrane domain followed by a soluble sulfatase-like catalytic domain in the periplasm. The atomic structure of the catalytic domain of EptC (cEptC) was crystallized and solved to a resolution of 2.40 A. cEptC adopts the alpha/beta/alpha fold of the sulfatase protein family and harbors a zinc-binding site. A phosphorylated Thr266 residue was observed that was hypothesized to mimic a covalent pEtN-enzyme intermediate. The requirement for Thr266 as well as the nearby residues Asn308, Ser309, His358 and His440 was ascertained via in vivo activity assays on mutant strains. The results establish a basis for the design of pEtN transferase inhibitors.
Crystallographic study of the phosphoethanolamine transferase EptC required for polymyxin resistance and motility in Campylobacter jejuni.,Fage CD, Brown DB, Boll JM, Keatinge-Clay AT, Trent MS Acta Crystallogr D Biol Crystallogr. 2014 Oct;70(Pt 10):2730-9. doi:, 10.1107/S1399004714017623. Epub 2014 Sep 27. PMID:25286856[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fage CD, Brown DB, Boll JM, Keatinge-Clay AT, Trent MS. Crystallographic study of the phosphoethanolamine transferase EptC required for polymyxin resistance and motility in Campylobacter jejuni. Acta Crystallogr D Biol Crystallogr. 2014 Oct;70(Pt 10):2730-9. doi:, 10.1107/S1399004714017623. Epub 2014 Sep 27. PMID:25286856 doi:http://dx.doi.org/10.1107/S1399004714017623
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