1k87

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[[Image:1k87.gif|left|200px]]
[[Image:1k87.gif|left|200px]]
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{{Structure
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|PDB= 1k87 |SIZE=350|CAPTION= <scene name='initialview01'>1k87</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1k87", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Proline_dehydrogenase Proline dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.8 1.5.99.8] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1k87| PDB=1k87 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k87 OCA], [http://www.ebi.ac.uk/pdbsum/1k87 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k87 RCSB]</span>
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}}
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'''Crystal structure of E.coli PutA (residues 1-669)'''
'''Crystal structure of E.coli PutA (residues 1-669)'''
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[[Category: Nadaria, S.]]
[[Category: Nadaria, S.]]
[[Category: Tanner, J J.]]
[[Category: Tanner, J J.]]
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[[Category: dimer]]
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[[Category: Dimer]]
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[[Category: multi-functional protein]]
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[[Category: Multi-functional protein]]
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[[Category: proline dehydrogenase]]
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[[Category: Proline dehydrogenase]]
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[[Category: shuttling]]
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[[Category: Shuttling]]
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[[Category: transcriptional repressor]]
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[[Category: Transcriptional repressor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:25:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:44:43 2008''
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Revision as of 19:25, 2 May 2008

Image:1k87.gif

Template:STRUCTURE 1k87

Crystal structure of E.coli PutA (residues 1-669)


Overview

The PutA flavoprotein from Escherichia coli plays multiple roles in proline catabolism by functioning as a membrane-associated bi-functional enzyme and a transcriptional repressor of proline utilization genes. The human homolog of the PutA proline dehydrogenase (PRODH) domain is critical in p53-mediated apoptosis and schizophrenia. Here we report the crystal structure of a 669-residue truncated form of PutA that shows both PRODH and DNA-binding activities, representing the first structure of a PutA protein and a PRODH enzyme from any organism. The structure is a domain-swapped dimer with each subunit comprising three domains: a helical dimerization arm, a 120-residue domain containing a three-helix bundle similar to that in the helix-turn-helix superfamily of DNA-binding proteins and a beta/alpha-barrel PRODH domain with a bound lactate inhibitor. Analysis of the structure provides insight into the mechanism of proline oxidation to pyrroline-5-carboxylate, and functional studies of a mutant protein suggest that the DNA-binding domain is located within the N-terminal 261 residues of E. coli PutA.

About this Structure

1K87 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein., Lee YH, Nadaraia S, Gu D, Becker DF, Tanner JJ, Nat Struct Biol. 2003 Feb;10(2):109-14. PMID:12514740 Page seeded by OCA on Fri May 2 22:25:13 2008

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