1k94
From Proteopedia
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'''Crystal structure of des(1-52)grancalcin with bound calcium''' | '''Crystal structure of des(1-52)grancalcin with bound calcium''' | ||
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[[Category: Jia, J.]] | [[Category: Jia, J.]] | ||
[[Category: Lollike, K.]] | [[Category: Lollike, K.]] | ||
| - | [[Category: | + | [[Category: Calcium binding protein]] |
| - | [[Category: | + | [[Category: Penta-ef-hand protein]] |
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Revision as of 19:27, 2 May 2008
Crystal structure of des(1-52)grancalcin with bound calcium
Overview
Grancalcin is a cytosolic Ca(2+)-binding protein originally identified in human neutrophils. It belongs to a new class of EF-hand proteins, called PEF proteins, which contain five EF-hand motifs. At the N-terminus of grancalcin there is a approximately 50 residue-long segment rich in glycines and prolines. The fifth EF-hand, unpaired within the monomer, provides a means for dimerization through pairing with its counterpart in a second molecule. The structure of full-length grancalcin in the apo form and with one EF3 within the dimer occupied by a Ca(2+) ion have been determined. Although the N-terminal segment was present in the molecule, this part was disordered in the crystals. Here, the structure of a truncated form of grancalcin, which is lacking 52 N-terminal residues, in the presence and absence of Ca(2+) is presented. In the Ca(2+)-bound form the ions are found in the EF1 and EF3 hands. Binding of Ca(2+) to these two EF hands produces only minor conformational changes, mostly within the EF1 Ca(2+)-binding loop. This observation supports the hypothesis, formulated on the basis of the structure of a homologous protein ALG-2 which shows significant differences in the orientation of EF4 and EF5 compared with grancalcin, that calcium is a necessary factor but not sufficient alone for inducing a significant conformational change in PEF proteins.
About this Structure
1K94 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of Ca(2+)-loaded human grancalcin., Jia J, Borregaard N, Lollike K, Cygler M, Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1843-9. Epub 2001, Nov 21. PMID:11717497 Page seeded by OCA on Fri May 2 22:27:29 2008
