1jan

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spinqualitylabelsall models 1jan, resolution 2.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

COMPLEX OF PRO-LEU-GLY-HYDROXYLAMINE WITH THE CATALYTIC DOMAIN OF MATRIX METALLO PROTEINASE-8 (PHE79 FORM)

Overview

For the collagenases PMNL-CL and FIB-CL, the presence of the N-terminal, Phe79 correlates with an increase in proteolytic activity. We have, determined the X-ray crystal structure of the recombinant Phe79-Gly242, catalytic domain of human neutrophil collagenase (PMNL-CL, MMP-8) using, the recently solved model of the Met80-Gly242 form for phasing and, subsequently refined it to a final crystallographic R-factor of 18.0% at, 2.5 A resolution. The PMNL-CL catalytic domain is a spherical molecule, with a flat active site cleft separating a smaller C-terminal subdomain, from a bigger N-terminal domain, that harbours two zinc ions, namely a, 'structural' and a 'catalytic' zinc, and two calcium ions. The N-terminal, segment prior to Pro86, which is disordered in the Met80-Gly242 form, packs against a concave hydrophobic surface made by the C-terminal helix., The N-terminal Phe79 ammonium group makes a salt link with the side chain, carboxylate group of the strictly conserved Asp232. Stabilization of the, catalytic site might be conferred via strong hydrogen bonds made by the, adjacent, likewise strictly conserved Asp233 with the characteristic, 'Met-turn', which forms the base of the active site residues.

About this Structure

1JAN is a Single protein structure of sequence from Homo sapiens with CA, ZN and HOA as ligands. Active as Neutrophil collagenase, with EC number 3.4.24.34 Full crystallographic information is available from OCA.

Reference

Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study., Reinemer P, Grams F, Huber R, Kleine T, Schnierer S, Piper M, Tschesche H, Bode W, FEBS Lett. 1994 Jan 31;338(2):227-33. PMID:8307185

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