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| | <StructureSection load='4u63' size='340' side='right'caption='[[4u63]], [[Resolution|resolution]] 1.67Å' scene=''> | | <StructureSection load='4u63' size='340' side='right'caption='[[4u63]], [[Resolution|resolution]] 1.67Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4u63]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrfc Agrfc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U63 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U63 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4u63]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_fabrum_str._C58 Agrobacterium fabrum str. C58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U63 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U63 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MHF:5,10-METHENYL-6,7,8-TRIHYDROFOLIC+ACID'>MHF</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MHF:5,10-METHENYL-6,7,8-TRIHYDROFOLIC+ACID'>MHF</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Atu1218 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 AGRFC])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u63 OCA], [https://pdbe.org/4u63 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u63 RCSB], [https://www.ebi.ac.uk/pdbsum/4u63 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u63 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u63 OCA], [http://pdbe.org/4u63 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u63 RCSB], [http://www.ebi.ac.uk/pdbsum/4u63 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u63 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PHRA_AGRT5 PHRA_AGRT5]] Photolyase involved in the repair of UV radiation-induced DNA damage. By using blue-light energy, catalyzes the photoreactivation of cyclobutane pyrimidine dimers (CPDs), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Can repair CPD lesions in ssDNA as well as in dsDNA.<ref>PMID:22066008</ref> | + | [https://www.uniprot.org/uniprot/PHRA_AGRFC PHRA_AGRFC] Photolyase involved in the repair of UV radiation-induced DNA damage. By using blue-light energy, catalyzes the photoreactivation of cyclobutane pyrimidine dimers (CPDs), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Can repair CPD lesions in ssDNA as well as in dsDNA.<ref>PMID:22066008</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Agrfc]] | + | [[Category: Agrobacterium fabrum str. C58]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kalms, J]] | + | [[Category: Kalms J]] |
| - | [[Category: Krauss, N]] | + | [[Category: Krauss N]] |
| - | [[Category: Lamparter, T]] | + | [[Category: Lamparter T]] |
| - | [[Category: Oberpichler, I]] | + | [[Category: Oberpichler I]] |
| - | [[Category: Scheerer, P]] | + | [[Category: Scheerer P]] |
| - | [[Category: Stetten, D von]]
| + | [[Category: Zhang F]] |
| - | [[Category: Zhang, F]] | + | [[Category: Von Stetten D]] |
| - | [[Category: Agrobacterium tumefacien]] | + | |
| - | [[Category: Cyclopyrimidine dimer]]
| + | |
| - | [[Category: Dna]]
| + | |
| - | [[Category: Dna damage]]
| + | |
| - | [[Category: Dna repair]]
| + | |
| - | [[Category: Double-stranded]]
| + | |
| - | [[Category: Fad]]
| + | |
| - | [[Category: Flavin]]
| + | |
| - | [[Category: Flavoprotein]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Methenyltetrahydrofolate]]
| + | |
| - | [[Category: Photolyase]]
| + | |
| - | [[Category: Photoreduction]]
| + | |
| - | [[Category: Trp triade]]
| + | |
| - | [[Category: Ultraviolet ray]]
| + | |
| Structural highlights
Function
PHRA_AGRFC Photolyase involved in the repair of UV radiation-induced DNA damage. By using blue-light energy, catalyzes the photoreactivation of cyclobutane pyrimidine dimers (CPDs), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Can repair CPD lesions in ssDNA as well as in dsDNA.[1]
Publication Abstract from PubMed
Photolyases are proteins with an FAD chromophore that repair UV-induced pyrimidine dimers on the DNA in a light dependent manner. The cyclobutane pyrimidine dimer (CPD) class III photolyases are structurally unknown but closely related to plant cryptochromes, which serve as blue-light photoreceptors. Here we present the crystal structure of a class III photolyase termed photolyase related protein A (PhrA) of Agrobacterium tumefaciens at resolution of 1.67 Angstrom. PhrA contains 5,10-methenyltetrahydrofolate (MTHF) as an antenna chromophore with a unique binding site and - mode. Two Trp residues play pivotal roles for stabilizing MTHF by a double pi-stacking sandwich. Plant cryptochrome I forms a pocket at the same site that could accommodate MTHF or a similar molecule. The PhrA structure and mutant studies showed that electrons flow during FAD photoreduction proceeds via two Trp-triads. The structural studies on PhrA give a clearer picture on the evolutionary transition from photolyase to photoreceptor.
The Class III Cyclobutane Pyrimidine Dimer Photolyase Structure Reveals a New Antenna Chromophore Binding Site and Alternative Photoreduction Pathways.,Scheerer P, Zhang F, Kalms J, von Stetten D, Krauss N, Oberpichler I, Lamparter T J Biol Chem. 2015 Mar 17. pii: jbc.M115.637868. PMID:25784552[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Oberpichler I, Pierik AJ, Wesslowski J, Pokorny R, Rosen R, Vugman M, Zhang F, Neubauer O, Ron EZ, Batschauer A, Lamparter T. A photolyase-like protein from Agrobacterium tumefaciens with an iron-sulfur cluster. PLoS One. 2011;6(10):e26775. doi: 10.1371/journal.pone.0026775. Epub 2011 Oct 31. PMID:22066008 doi:http://dx.doi.org/10.1371/journal.pone.0026775
- ↑ Scheerer P, Zhang F, Kalms J, von Stetten D, Krauss N, Oberpichler I, Lamparter T. The Class III Cyclobutane Pyrimidine Dimer Photolyase Structure Reveals a New Antenna Chromophore Binding Site and Alternative Photoreduction Pathways. J Biol Chem. 2015 Mar 17. pii: jbc.M115.637868. PMID:25784552 doi:http://dx.doi.org/10.1074/jbc.M115.637868
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