7un7
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==DNA Polymerase lambda in complex with a 1nt microhomology substrate== | |
| + | <StructureSection load='7un7' size='340' side='right'caption='[[7un7]], [[Resolution|resolution]] 2.04Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7un7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UN7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7un7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7un7 OCA], [https://pdbe.org/7un7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7un7 RCSB], [https://www.ebi.ac.uk/pdbsum/7un7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7un7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The double-strand break (DSB) repair pathway called microhomology-mediated end-joining (MMEJ) is thought to be dependent on DNA polymerase theta (Poltheta) and occur independently of nonhomologous end-joining (NHEJ) factors. An unresolved question is whether MMEJ is facilitated by a single Poltheta-mediated end-joining pathway or consists of additional undiscovered pathways. We find that human X-family Pollambda, which functions in NHEJ, additionally exhibits robust MMEJ activity like Poltheta. Pollambda promotes MMEJ in mammalian cells independently of essential NHEJ factors LIG4/XRCC4 and Poltheta, which reveals a distinct Pollambda-dependent MMEJ mechanism. X-ray crystallography employing in situ photo-induced DSB formation captured Pollambda in the act of stabilizing a microhomology-mediated DNA synapse with incoming nucleotide at 2.0 A resolution and reveals how Pollambda performs replication across a DNA synapse joined by minimal base-pairing. Last, we find that Pollambda is semisynthetic lethal with BRCA1 and BRCA2. Together, these studies indicate Pollambda MMEJ as a distinct DSB repair mechanism. | ||
| - | + | Pollambda promotes microhomology-mediated end-joining.,Chandramouly G, Jamsen J, Borisonnik N, Tyagi M, Calbert ML, Tredinnick T, Ozdemir AY, Kent T, Demidova EV, Arora S, Wilson SH, Pomerantz RT Nat Struct Mol Biol. 2023 Jan;30(1):107-114. doi: 10.1038/s41594-022-00895-4. , Epub 2022 Dec 19. PMID:36536104<ref>PMID:36536104</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7un7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Jamsen JA]] | ||
Revision as of 06:41, 29 March 2023
DNA Polymerase lambda in complex with a 1nt microhomology substrate
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