6ybe
From Proteopedia
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==RNASE 3/1 version2== | ==RNASE 3/1 version2== | ||
| - | <StructureSection load='6ybe' size='340' side='right'caption='[[6ybe]]' scene=''> | + | <StructureSection load='6ybe' size='340' side='right'caption='[[6ybe]], [[Resolution|resolution]] 1.14Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YBE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ybe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YBE FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ybe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ybe OCA], [https://pdbe.org/6ybe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ybe RCSB], [https://www.ebi.ac.uk/pdbsum/6ybe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ybe ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ybe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ybe OCA], [https://pdbe.org/6ybe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ybe RCSB], [https://www.ebi.ac.uk/pdbsum/6ybe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ybe ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Design of novel antibiotics to fight antimicrobial resistance is one of the first global health priorities. Novel protein-based strategies come out as alternative therapies. Based on the structure-function knowledge of the RNase A superfamily we have engineered a chimera that combines RNase 1 highest catalytic activity with RNase 3 unique antipathogen properties. A first construct (RNase 3/1-v1) was successfully designed with a catalytic activity 40-fold higher than RNase 3, but alas in detriment of its anti-pathogenic activity. Next, two new versions of the original chimeric protein were created showing improvement in the antimicrobial activity. Both second generation versions (RNases 3/1-v2 and -v3) incorporated a loop characteristic of RNase 3 (L7), associated to antimicrobial activity. Last, removal of an RNase 1 flexible loop (L1) in the third version enhanced its antimicrobial properties and catalytic efficiency. Here we solved the 3D structures of the three chimeras at atomic resolution by X-ray crystallography. Structural analysis outlined the key functional regions. Prediction by molecular docking of the protein chimera in complex with dinucleotides highlighted the contribution of the C-terminal region to shape the substrate binding cavity and determine the base selectivity and catalytic efficiency. Nonetheless, the structures that incorporated the key features related to RNase 3 antimicrobial activity retained the overall RNase 1 active site conformation together with the essential structural elements for binding to the human ribonuclease inhibitor (RNHI), ensuring non-cytotoxicity. Results will guide us in the design of the best RNase pharmacophore for anti-infective therapies. | ||
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| + | Exploring the RNase A scaffold to combine catalytic and antimicrobial activities. Structural characterization of RNase 3/1 chimeras.,Fernandez-Millan P, Vazquez-Monteagudo S, Boix E, Prats-Ejarque G Front Mol Biosci. 2022 Sep 14;9:964717. doi: 10.3389/fmolb.2022.964717. , eCollection 2022. PMID:36188223<ref>PMID:36188223</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ybe" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
[[Category: Boix E]] | [[Category: Boix E]] | ||
[[Category: Fernandez-Millan P]] | [[Category: Fernandez-Millan P]] | ||
[[Category: Prats-Ejarque G]] | [[Category: Prats-Ejarque G]] | ||
[[Category: Vazquez-Monteagudo S]] | [[Category: Vazquez-Monteagudo S]] | ||
Revision as of 06:59, 29 March 2023
RNASE 3/1 version2
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