7qv8
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Leishmania infantum BRC1 repeat in complex with LiRAD51== |
| - | <StructureSection load='7qv8' size='340' side='right'caption='[[7qv8]]' scene=''> | + | <StructureSection load='7qv8' size='340' side='right'caption='[[7qv8]], [[Resolution|resolution]] 2.15Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7qv8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_infantum Leishmania infantum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QV8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qv8 OCA], [https://pdbe.org/7qv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qv8 RCSB], [https://www.ebi.ac.uk/pdbsum/7qv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qv8 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qv8 OCA], [https://pdbe.org/7qv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qv8 RCSB], [https://www.ebi.ac.uk/pdbsum/7qv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qv8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A4I3C9_LEIIN A4I3C9_LEIIN] Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA.[RuleBase:RU364139] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Interaction of BRCA2 through ca. 30 amino acid residue motifs, BRC repeats, with RAD51 is a conserved feature of the double-strand DNA break repair by homologous recombination in eukaryotes. In humans the binding of the eight BRC repeats is defined by two sequence motifs, FxxA and LFDE, interacting with distinct sites on RAD51. Little is known of the interaction of BRC repeats in other species, especially in protozoans, where variable number of BRC repeats are found in BRCA2 proteins. Here, we have studied in detail the interactions of the two BRC repeats in Leishmania infantum BRCA2 with RAD51. We show LiBRC1 is a high-affinity repeat and determine the crystal structure of its complex with LiRAD51. Using truncation mutagenesis of the LiBRC1 repeat, we demonstrate that high affinity binding is maintained in the absence of an LFDE-like motif and suggest compensatory structural features. These observations point towards a divergent evolution of BRC repeats, where a common FxxA-binding ancestor evolved additional contacts for affinity maturation and fine-tuning. | ||
| + | |||
| + | Divergent binding mode for a protozoan BRC repeat to RAD51.,Pantelejevs T, Hyvonen M Biochem J. 2022 May 27;479(10):1031-1043. doi: 10.1042/BCJ20220141. PMID:35502837<ref>PMID:35502837</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7qv8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Leishmania infantum]] |
| + | [[Category: Hyvonen M]] | ||
| + | [[Category: Pantelejevs T]] | ||
Revision as of 07:02, 29 March 2023
Leishmania infantum BRC1 repeat in complex with LiRAD51
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