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| | <StructureSection load='4ure' size='340' side='right'caption='[[4ure]], [[Resolution|resolution]] 1.40Å' scene=''> | | <StructureSection load='4ure' size='340' side='right'caption='[[4ure]], [[Resolution|resolution]] 1.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ure]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aroae Aroae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4URE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4URE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ure]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aromatoleum_aromaticum_EbN1 Aromatoleum aromaticum EbN1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4URE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4URE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PS:3-PYRIDINIUM-1-YLPROPANE-1-SULFONATE'>1PS</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PS:3-PYRIDINIUM-1-YLPROPANE-1-SULFONATE'>1PS</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4urf|4urf]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ure FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ure OCA], [https://pdbe.org/4ure PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ure RCSB], [https://www.ebi.ac.uk/pdbsum/4ure PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ure ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(S)-1-phenylethanol_dehydrogenase (S)-1-phenylethanol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.311 1.1.1.311] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ure FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ure OCA], [http://pdbe.org/4ure PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ure RCSB], [http://www.ebi.ac.uk/pdbsum/4ure PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ure ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5P8S7_AROAE Q5P8S7_AROAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aroae]] | + | [[Category: Aromatoleum aromaticum EbN1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Breuer, M]] | + | [[Category: Breuer M]] |
| - | [[Category: Buesing, I]] | + | [[Category: Buesing I]] |
| - | [[Category: Hauer, B]] | + | [[Category: Hauer B]] |
| - | [[Category: Hoeffken, H W]] | + | [[Category: Hoeffken HW]] |
| - | [[Category: Rabus, R]] | + | [[Category: Rabus R]] |
| - | [[Category: Woehlbrand, L]] | + | [[Category: Woehlbrand L]] |
| - | [[Category: Alcohol dehydrogenase]]
| + | |
| - | [[Category: Anaerobic degradation]]
| + | |
| - | [[Category: Aromatic compound]]
| + | |
| - | [[Category: Molecular genetic]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Short chain dehydrogenase]]
| + | |
| - | [[Category: Stereochemistry]]
| + | |
| Structural highlights
Function
Q5P8S7_AROAE
Publication Abstract from PubMed
The dehydrogenation of 1-(4-hydroxyphenyl)-ethanol to 4-hydroxyacetophenone represents the second reaction step during anaerobic degradation of p-ethylphenol in the denitrifying bacterium 'Aromatoleum aromaticum' EbN1. Previous proteogenomic studies identified two different proteins (ChnA and EbA309) as possible candidates for catalyzing this reaction [Wohlbrand et al: J Bacteriol 2008;190:5699-5709]. Physiological-molecular characterization of newly generated unmarked in-frame deletion and complementation mutants allowed defining ChnA (renamed here as Hped) as the enzyme responsible for 1-(4-hydroxyphenyl)-ethanol oxidation. Hped [1-(4-hydroxyphenyl)-ethanol dehydrogenase] belongs to the 'classical' family within the short-chain alcohol dehydrogenase/reductase (SDR) superfamily. Hped was overproduced in Escherichia coli, purified and crystallized. The X-ray structures of the apo- and NAD+-soaked form were resolved at 1.5 and 1.1 A, respectively, and revealed Hped as a typical homotetrameric SDR. Modeling of the substrate 4-hydroxyacetophenone (reductive direction of Hped) into the active site revealed the structural determinants of the strict (R)-specificity of Hped (Phe187), contrasting the (S)-specificity of previously reported 1-phenylethanol dehydrogenase (Ped; Tyr93) from strain EbN1 [Hoffken et al: Biochemistry 2006;45:82-93].
Molecular Genetic and Crystal Structural Analysis of 1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase from 'Aromatoleum aromaticum' EbN1.,Busing I, Hoffken HW, Breuer M, Wohlbrand L, Hauer B, Rabus R J Mol Microbiol Biotechnol. 2015;25(5):327-39. doi: 10.1159/000439113. Epub 2015 , Oct 22. PMID:26488297[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Busing I, Hoffken HW, Breuer M, Wohlbrand L, Hauer B, Rabus R. Molecular Genetic and Crystal Structural Analysis of 1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase from 'Aromatoleum aromaticum' EbN1. J Mol Microbiol Biotechnol. 2015;25(5):327-39. doi: 10.1159/000439113. Epub 2015 , Oct 22. PMID:26488297 doi:http://dx.doi.org/10.1159/000439113
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