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| | <StructureSection load='4v4a' size='340' side='right'caption='[[4v4a]], [[Resolution|resolution]] 9.50Å' scene=''> | | <StructureSection load='4v4a' size='340' side='right'caption='[[4v4a]], [[Resolution|resolution]] 9.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4v4a]] is a 53 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pnx 1pnx] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pny 1pny]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4v4a]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pnx 1pnx] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pny 1pny]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V4A FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pns|1pns]], [[1pnu|1pnu]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4a OCA], [https://pdbe.org/4v4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v4a RCSB], [https://www.ebi.ac.uk/pdbsum/4v4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4a ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4a OCA], [http://pdbe.org/4v4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v4a RCSB], [http://www.ebi.ac.uk/pdbsum/4v4a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4a ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cate, J H.D]] | + | [[Category: Cate JHD]] |
| - | [[Category: Ridgeway, W K]] | + | [[Category: Ridgeway WK]] |
| - | [[Category: Santoso, S]] | + | [[Category: Santoso S]] |
| - | [[Category: Seymaner, V]] | + | [[Category: Seymaner V]] |
| - | [[Category: Vila-Sanjurjo, A]] | + | [[Category: Vila-Sanjurjo A]] |
| - | [[Category: Yu, K]] | + | [[Category: Yu K]] |
| - | [[Category: Zhang, W]] | + | [[Category: Zhang W]] |
| - | [[Category: 30s ribosomal subunit]]
| + | |
| - | [[Category: Protein-protein complex]]
| + | |
| - | [[Category: Protein-rna complex]]
| + | |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Rna-rna complex]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis.
X-ray crystal structures of the WT and a hyper-accurate ribosome from Escherichia coli.,Vila-Sanjurjo A, Ridgeway WK, Seymaner V, Zhang W, Santoso S, Yu K, Cate JH Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8682-7. Epub 2003 Jul 9. PMID:12853578[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Vila-Sanjurjo A, Ridgeway WK, Seymaner V, Zhang W, Santoso S, Yu K, Cate JH. X-ray crystal structures of the WT and a hyper-accurate ribosome from Escherichia coli. Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8682-7. Epub 2003 Jul 9. PMID:12853578 doi:10.1073/pnas.1133380100
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