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<scene name='95/952717/Mras/2'>MRAS</scene>
<scene name='95/952717/Mras/2'>MRAS</scene>
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<jmol>
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MRAS is a membrane bound structure that aids the complex in localizing near other structures such as the RAS-RAF-MAPK complex in order to initiate downstream signaling. In its inactive state, MRAS is bound to GDP. When signaled by growth factors, the GDP is exchanged for GTP. The now <scene name='95/952718/Zoom_in_gtp/1'>BTP bound MRAS</scene> undergoes a conformational change of the switch I and switch II regions. This conformational change activates the protein allowing it to bind more easily with the SHOC2-PP1C complex. In comparison to other RAS proteins, MRAS has a greater affinity for the SHOC2-PP1C complex.
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<script>
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load /wiki/scripts/95/952718/Zoom_out/1.spt
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load /wiki/scripts/95/952718/Zoom_in/1.spt
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<load /wiki/scripts/95/952718/Zoom_in_gtp/1.spt>
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</script>
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<text>GTP bound MRAS</text>
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</jmolLink>
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</jmol>
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<scene name='95/952718/Zoom_out/1'>Text To Be Displayed</scene>
 
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<scene name='95/952718/Zoom_in/1'>Text To Be Displayed</scene>
 
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<scene name='95/952718/Zoom_in_gtp/1'>Text To Be Displayed</scene>
 
=== Key Ligand Interactions ===
=== Key Ligand Interactions ===
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[[Image:Amphbindingfinal.png|275 px|right|thumb|'''Figure 3''': Electrostatic illustration of the amphipathic binding pocket of the LPA<sub>1</sub> receptor. This binding pocket was revealed by cutting away the exterior or the protein. This binding pocket, located in the interior of the protein, has both polar and nonpolar regions. The blue and red coloration highlight the positively and negatively charged regions, respectively, and the white color shows the nonpolar region of the binding pocket.]]
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=== SHOC2 and PP1C ===
=== SHOC2 and PP1C ===
PP1C binds to SHOC2 on its leucine rich region(LRR). Specifically, on two broad surfaces between LRR2 and LRR5 and between LRR7 and LRR11. Five main hydrogen bonds are made: E56-R182, E167-R203, E54-K180, R187-H178, R188-E155. The binding regions can also be shown as acidic and basic patches on <scene name='95/952718/Acid_base_pp1c/1'>PP1C</scene> and <scene name='95/952718/Acid_base_shoc2/1'>SHOC2</scene>. The corresponding patches interact to form a <scene name='95/952718/Acid_base_shoc2pp1c/1'>binary complex</scene>.
PP1C binds to SHOC2 on its leucine rich region(LRR). Specifically, on two broad surfaces between LRR2 and LRR5 and between LRR7 and LRR11. Five main hydrogen bonds are made: E56-R182, E167-R203, E54-K180, R187-H178, R188-E155. The binding regions can also be shown as acidic and basic patches on <scene name='95/952718/Acid_base_pp1c/1'>PP1C</scene> and <scene name='95/952718/Acid_base_shoc2/1'>SHOC2</scene>. The corresponding patches interact to form a <scene name='95/952718/Acid_base_shoc2pp1c/1'>binary complex</scene>.
=== SHOC2 and MRAS ===
=== SHOC2 and MRAS ===
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MRAS is initially bound to GDP causing it to be in its inactive state. This form cannot bind to the SHOC2-PP1C complex due to steric clashing. Once GDP is exchanged for GTP to activate the protein, conformational changed occur withing the switch I and switch II regions to allow MRAS to interact with SHOC2. These interactions include hydrogen bonds of and pi stacking. The primary hydrogen bonds are R288-Q71 and R177-E47. Pi staking occurs at R104-R83.
=== PP1C and MRAS ===
=== PP1C and MRAS ===
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<references/>
<references/>
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==Proteopedia Resources==
 
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[http://proteopedia.org/wiki/index.php/Category:Lysophosphatidic_acid_binding Category:Lysophosphatidic acid binding]
 
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[http://proteopedia.org/wiki/index.php/Category:Lysophosphatidic_acid Category:Lysophosphatidic acid]
 
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[http://proteopedia.org/wiki/index.php/User:R._Jeremy_Johnson/CH462:Biochemistry_II_Butler_University Butler University Proteopedia Pages]
 
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See also:
 
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*[[Receptor]]
 
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*[[Transmembrane (cell surface) receptors]]
 
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*[[G protein-coupled receptors]]
 
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</StructureSection>
 
==Student Contributors==
==Student Contributors==
Madeline Gilbert
Madeline Gilbert

Revision as of 16:42, 6 April 2023

This page, as it appeared on March 17, 2023, was featured in this article in the journal Biochemistry and Molecular Biology Education.

SHOC2-PP1C-MRAS

SHOC2-MRAS-PP1C

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