Introduction

Immunoglobulin M, or IgM, is one of multiple types of immunoglobulins that exist in humans. IgM presents itself on the surface of a B cell to act as a B Cell Receptor (BCR). Upon the binding of an antigen to the BCR, the B cell will activate, proliferate, and produce other Ig compounds. These include IgG, IgD, IgA, and IgE antibodies, which all have specific roles in the various forms of immune response. Because of this, activation of the IgM BCR is a critical step in the beginning of an immune response.

The structure of IgM was determined using Cryo-EM to visualize the atoms within the protein. However, due to poor resolution within specific regions of the IgM BCR, not every atom has been able to be visualized.

Structure

The IgM BCR consists of six separate chains (Figure 1) that make up three main domains in the molecule. A depiction of the IgM shows two heavy and two light chains that together form the Fab region, or variable fragment at the top of the molecule where the antigen binding sites are located. The two heavy chains extend below the Fab region through the Fc region and eventually connect to the Igα/β heterodimer to form the transmembrane region which anchors the overall complex to the B cell. The overall structure, expression, and function of the IgM BCR has been found to be strongly influenced by the transmembrane region in which Ig α/β interactions as a heterodimer influence cell surface expression, receptor assembly, and effective signal transduction (Tolar and Dylke citation). In each domain, interactions between individual chains are important to understand the complex as a whole. All future 3D depictions will be as in Figure 1.

Figure 1. IgM BCR Structure Overview. Depiction of the IgM BCR expressed on the membrane of a B cell. Includes all major components including the α/β heterodimer, heavy and light chains, antigen binding sites, and the ITAM region for signal transduction.

Transmembrane Region

The IgM BCR is anchored to B-cell membranes through the which is broken up into both extracellular and integral domains which sit on top of or span through the membrane, respectively (Figure 1). IgM BCR assembly requires dimerization of the Igα and Igβ subunits which embed within the B-cell membrane. (Tolar citation) The dimerizes within the extracellular region with a . Additional dimerization is believed to occur within the integral region via a hydrogen bond; the involved residues and interaction have not been confirmed. Although the mechanism of disulfide bridge formation is still unknown, it is believed that via N-linked asparagine glycosylation (NAGs) on various residues in the extracellular region of both the Igα and and Igβ chains help facilitate this process. Chaperone proteins are typically bound to the alpha and beta subunits until dimerization occurs; at this point the rest of the BCR complex can be recruited. (Dylke citation)

After Igα and Igβ dimerization, the transmembrane helices of the heavy chains can embed within the B-cell membrane. (Tolar citation) The side chains of this are primarily hydrophobic side chains that allow for interactions with the hydrophobic tails in the phospholipid bilayer. The 4 helices (Figure 2) are primarily held together through hydrophobic interactions; however, a a few polar residues are included on the interior of the helix structure which interact with a few polar residues on the Igα and Igβ chains. (Dylke citation)

Furthermore, both the Igα and Igβ chains have cytoplasmic tails that extend into the B cell (Figure 1). Each of these tails contain a immuno-receptor tyrosine-based activation motif (ITAM) region to facilitate signal transduction (Figure 4). (Ma citation)

Figure 2. 4-pass integral helix. Pymol image of the integral helices in IgM BCR (PDB:7xq8) rotated on the x and y axes. Side chains are shown as sticks. Brown=Ig alpha, orange=Ig beta, pink=heavy chain A, blue=heavy chain B.

Fc Region

The constant region of IgM is made up of the 2 . These heavy chains form a bridge to connect the Fab fragment, or variable region, to the transmembrane region. They also act as a wire to allow the variable region to send a cellular signal through to the intermembrane region once an antigen has been bound.

help to stabilize and hold the heavy chains and Ig Alpha/Beta chains together in the extracellular portion of the intermembrane region.

Fab Region

Because the Fab region of IgM is so poorly resolved, a structural comparison to another antibody was performed to approximate where an antigen would bind to the . Figure 2 shows the antigen binding motif (located on the Fab region) of an Ig-BCR complex that was engineered to contain the variable region of a neutralizing antibody called VCR01, an antibody that targets the epitope of HIV molecules. It contains areas referred to as complementary-determining regions, or CDRs, which are where the antigen makes contact with the antibody on the Fab domain. Showing them as surface representation allows us to make structural comparisons to the IgM antibody, and to highlight their similarities. The CDRs are similarly placed within the heavy and light chain variable regions between both antibodies. It is speculated that they are structurally similar because the VCR01 antibody can effectively target multiple HIV strains while IgM is the preliminary antibody produced and released during early stages of the immune response, thus it is able to respond in larger concentrations while antibodies that are more specific to the antigen are being produced.

Due to the Fab region of the IgM antibody being poorly resolved, the specific side chain interactions between the heavy and light chains have not been determined. This depiction of the shows how the 4 β-sandwiches fit together; heavy chain A and B of the Fab region form a complex with the rest of the molecule via interactions with the heavy A and B of the Fc region, before continuing down into the intracellular domain to interact with the transmembrane region. The light chains however are only connected to the complex by forming interactions with the heavy chains within the Fab region. Although the specific residues within the Fab region have yet to be identified, it is estimated that each β-sandwich contains one disulfide bridge with additional hydrogen bonds.

Figure 3. Surface Representation of IgM Antibody Binding Pocket.

Signal Transduction

The diagram in Figure 4 depicts the initial process of B cell activation by the antigen binding to the antibody at the Fab region. The underlying mechanism for signal transduction is unknown but it is speculated to operate under what is known as the conserved assembly mechanism. This means that upon antigen binding, BCRs on the surface of the cell begin to cluster to cause the phosphorylation of the immunoreceptor tyrosine-based activation motifs located in Igα and Igβ. In its “off” state, the constant region 4 of heavy chain B overlaps the extracellular components of Igα and Igβ. As the antigen binds, it induces a conformational change to release the overlap and allow for clustering about the BCR. Now, in its “on” state the phosphorylation of the ITAM region (observed here as the conserved tyrosine residues are phosphorylated) within the intracellular tails of Igα and Igβ drives downstream kinase activity to continue to process of signal cascading.

Figure 4. IgM Antibody Signal Transduction following Antigen Binding.