1kf4

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[[Image:1kf4.jpg|left|200px]]
[[Image:1kf4.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1kf4 |SIZE=350|CAPTION= <scene name='initialview01'>1kf4</scene>, resolution 1.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1kf4", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1kf4| PDB=1kf4 | SCENE= }}
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|RELATEDENTRY=[[1kf2|1KF2]], [[1kf3|1KF3]], [[1kf5|1KF5]], [[1kf7|1KF7]], [[1kf8|1KF8]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kf4 OCA], [http://www.ebi.ac.uk/pdbsum/1kf4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kf4 RCSB]</span>
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}}
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'''Atomic Resolution Structure of RNase A at pH 6.3'''
'''Atomic Resolution Structure of RNase A at pH 6.3'''
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[[Category: Wilson, K S.]]
[[Category: Wilson, K S.]]
[[Category: Zagari, A.]]
[[Category: Zagari, A.]]
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[[Category: crystal]]
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[[Category: Crystal]]
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[[Category: ph]]
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[[Category: Ph]]
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[[Category: rnase some]]
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[[Category: Rnase some]]
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[[Category: soaking]]
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[[Category: Soaking]]
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[[Category: titration]]
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[[Category: Titration]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:40:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:47:40 2008''
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Revision as of 19:40, 2 May 2008

Image:1kf4.jpg

Template:STRUCTURE 1kf4

Atomic Resolution Structure of RNase A at pH 6.3


Overview

The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.

About this Structure

1KF4 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829 Page seeded by OCA on Fri May 2 22:40:09 2008

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