7uz2
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of beta-glycosidase from Sulfolobus solfataricus in complex with C5a-fluoro-valienide.== | |
| + | <StructureSection load='7uz2' size='340' side='right'caption='[[7uz2]], [[Resolution|resolution]] 1.83Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7uz2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UZ2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OML:(1R,2S,3R,4R)-5-fluoro-6-(hydroxymethyl)cyclohex-5-ene-1,2,3,4-tetrol'>OML</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uz2 OCA], [https://pdbe.org/7uz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uz2 RCSB], [https://www.ebi.ac.uk/pdbsum/7uz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uz2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0E3K5E4_SACSO A0A0E3K5E4_SACSO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Suitably configured allyl ethers of unsaturated cyclitols act as substrates of beta-glycosidases, reacting via allylic cation transition states. Incorporation of halogens at the vinylic position of these carbasugars, along with an activated leaving group, generates potent inactivators of beta-glycosidases. Enzymatic turnover of these halogenated cyclitols (F, Cl, Br) displayed a counter-intuitive trend wherein the most electronegative substituents yielded the most labile pseudo-glycosidic linkages. Structures of complexes with the Sulfolobus beta-glucosidase revealed similar enzyme-ligand interactions to those seen in complexes with a 2-fluorosugar inhibitor, the lone exception being displacement of tyrosine 322 from the active site by the halogen. Mutation of Y322 to Y322F largely abolished glycosidase activity, consistent with lost interactions at O5, but minimally affected (7-fold) rates of carbasugar hydrolysis, yielding a more selective enzyme for unsaturated cyclitol ether hydrolysis. | ||
| - | + | Vinyl Halide-Modified Unsaturated Cyclitols are Mechanism-Based Glycosidase Inhibitors.,Danby PM, Jeong A, Sim L, Sweeney RP, Wardman JF, Karimi R, Geissner A, Worrall LJ, Reid JP, Strynadka NCJ, Withers S Angew Chem Int Ed Engl. 2023 Mar 20:e202301258. doi: 10.1002/anie.202301258. PMID:36940280<ref>PMID:36940280</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7uz2" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Sim | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Wardman | + | [[Category: Saccharolobus solfataricus]] |
| - | [[Category: | + | [[Category: Danby PM]] |
| - | [[Category: | + | [[Category: Geissner A]] |
| + | [[Category: Jeong A]] | ||
| + | [[Category: Sim L]] | ||
| + | [[Category: Strynadka NCJ]] | ||
| + | [[Category: Sweeney RP]] | ||
| + | [[Category: Wardman JF]] | ||
| + | [[Category: Withers SG]] | ||
| + | [[Category: Worrall LJ]] | ||
Revision as of 06:22, 7 April 2023
Structure of beta-glycosidase from Sulfolobus solfataricus in complex with C5a-fluoro-valienide.
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