This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1kfa
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1kfa.jpg|left|200px]] | [[Image:1kfa.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1kfa", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_1kfa| PDB=1kfa | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''Crystal structure of Fab fragment complexed with gibberellin A4''' | '''Crystal structure of Fab fragment complexed with gibberellin A4''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFA OCA]. | |
==Reference== | ==Reference== | ||
Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12054627 12054627] | Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12054627 12054627] | ||
| - | [[Category: Homo sapiens]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Asami, O.]] | [[Category: Asami, O.]] | ||
[[Category: Fushinobu, S.]] | [[Category: Fushinobu, S.]] | ||
| Line 32: | Line 27: | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
[[Category: Yamaguchi, I.]] | [[Category: Yamaguchi, I.]] | ||
| - | [[Category: | + | [[Category: Immunoglobuiln fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:40:30 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:40, 2 May 2008
Crystal structure of Fab fragment complexed with gibberellin A4
Overview
Gibberellins, a class of plant hormones, consist of more than 120 members. Only a few of them are recognized by a receptor that remains unknown. The haptenic mouse monoclonal antibody, 4-B8(8)/E9, was generated against gibberellin A(4) (GA(4)) to recognize biologically active GA selectivity, and we attempted to confirm the binding properties between the antibody and GA(4). We carried out an X-ray crystallographic analysis of the 4-B8(8)/E9 Fab fragment complexed with GA(4) at a 2.8 A resolution by using the molecular replacement method. The crystal structure of the Fab fragment showed the typical immunoglobulin fold of the beta-barrel structure which is the common motif of all antibodies. A small hapten-combining site was made up of three heavy chain CDR loops. On the other hand, CDRs of the light chain did not interact directly with GA(4). The C/D rings of the GA(4) molecule were in van der Waals contact mainly with the aromatic side chain of Tyr100AH and Phe100BH of CDR-H3. The 3 beta-hydroxyl and 6 beta-carboxyl groups were, respectively, hydrogen-bonded to the main chain of Ala33H and to the Thr53H heavy chain.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment., Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I, Biochem Biophys Res Commun. 2002 Apr 26;293(1):489-96. PMID:12054627 Page seeded by OCA on Fri May 2 22:40:30 2008
