8c9v
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==O-methyltransferase from Desulfuromonas acetoxidans== | |
| + | <StructureSection load='8c9v' size='340' side='right'caption='[[8c9v]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8c9v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfuromonas_acetoxidans_DSM_684 Desulfuromonas acetoxidans DSM 684]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C9V FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c9v OCA], [https://pdbe.org/8c9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c9v RCSB], [https://www.ebi.ac.uk/pdbsum/8c9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c9v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q1JXV1_DESA6 Q1JXV1_DESA6] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Oxygen-directed methylation is a ubiquitous tailoring reaction in natural product pathways catalysed by O-methyltransferases (OMTs). Promiscuous OMT biocatalysts are thus a valuable asset in the toolkit for sustainable synthesis and optimization of known bioactive scaffolds for drug development. Here, we characterized the enzymatic properties and substrate scope of two bacterial OMTs from Desulforomonas acetoxidans and Streptomyces avermitilis and determined their crystal structures. Both OMTs methylated a wide range of catechol-like substrates, including flavonoids, coumarins, hydroxybenzoic acids, and their respective aldehydes, an anthraquinone and an indole. One enzyme also accepted a steroid. The product range included pharmaceutically relevant compounds such as (iso)fraxidin, iso(scopoletin), chrysoeriol, alizarin 1-methyl ether, and 2-methoxyestradiol. Interestingly, certain non-catechol flavonoids and hydroxybenzoic acids were also methylated. This study expands the knowledge on substrate preference and structural diversity of bacterial catechol OMTs and paves the way for their use in (combinatorial) pathway engineering. | ||
| - | + | Structural Characterization and Extended Substrate Scope Analysis of Two Mg(2+) -Dependent O-Methyltransferases from Bacteria.,Sokolova N, Zhang L, Deravi S, Oerlemans R, Groves MR, Haslinger K Chembiochem. 2023 Mar 21:e202300076. doi: 10.1002/cbic.202300076. PMID:36942619<ref>PMID:36942619</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8c9v" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Desulfuromonas acetoxidans DSM 684]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Groves MR]] | ||
| + | [[Category: Zhang L]] | ||
Revision as of 06:28, 7 April 2023
O-methyltransferase from Desulfuromonas acetoxidans
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