7djt
From Proteopedia
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==Human SARM1 inhibitory state bounded with inhibitor dHNN== | ==Human SARM1 inhibitory state bounded with inhibitor dHNN== | ||
| - | <StructureSection load='7djt' size='340' side='right'caption='[[7djt]]' scene=''> | + | <StructureSection load='7djt' size='340' side='right'caption='[[7djt]], [[Resolution|resolution]] 2.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DJT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7djt]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DJT FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7djt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7djt OCA], [https://pdbe.org/7djt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7djt RCSB], [https://www.ebi.ac.uk/pdbsum/7djt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7djt ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=H8L:O3-methyl+O5-(2-methylpropyl)+2,6-dimethyl-4-[2-(oxidanylamino)phenyl]pyridine-3,5-dicarboxylate'>H8L</scene>, <scene name='pdbligand=VI3:(2~{R})-2-azanyl-3-[bis(oxidanylidene)-$l^{5}-sulfanyl]propanoic+acid'>VI3</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7djt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7djt OCA], [https://pdbe.org/7djt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7djt RCSB], [https://www.ebi.ac.uk/pdbsum/7djt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7djt ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SARM1_HUMAN SARM1_HUMAN] Negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway which plays a pivotal role in activating axonal degeneration following injury. Promotes Wallerian degeneration an injury-induced axonal death pathway which involves degeneration of an axon distal to the injury site. Can activate neuronal death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response. Inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.<ref>PMID:15123841</ref> <ref>PMID:16964262</ref> <ref>PMID:16985498</ref> <ref>PMID:20306472</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | SARM1 regulates axonal degeneration through its NAD-metabolizing activity and is a drug target for neurodegenerative disorders. We designed and synthesized fluorescent conjugates of styryl derivative with pyridine to serve as substrates of SARM1, which exhibited large red-shifts after conversion. With the conjugates, SARM1 activation was visualized in live cells following elevation of endogenous NMN or treatment with a cell-permeant NMN-analog. In neurons, imaging documented mouse SARM1 activation preceded vincristine-induced axonal degeneration by hours. Library screening identified a derivative of nisoldipine as a covalent inhibitor of SARM1 that reacted with the cysteines, especially Cys311 in its ARM domain and blocked its NMN-activation, protecting axons from degeneration. The Cryo-EM structure showed that SARM1 was locked into an inactive conformation by the inhibitor, uncovering a potential neuroprotective mechanism of dihydropyridines. | ||
| + | |||
| + | Permeant fluorescent probes visualize the activation of SARM1 and uncover an anti-neurodegenerative drug candidate.,Li WH, Huang K, Cai Y, Wang QW, Zhu WJ, Hou YN, Wang S, Cao S, Zhao ZY, Xie XJ, Du Y, Lee CS, Lee HC, Zhang H, Zhao YJ Elife. 2021 May 4;10. pii: 67381. doi: 10.7554/eLife.67381. PMID:33944777<ref>PMID:33944777</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7djt" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[SARM1 3D structures|SARM1 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cai Y]] | [[Category: Cai Y]] | ||
[[Category: Zhang H]] | [[Category: Zhang H]] | ||
Revision as of 06:36, 7 April 2023
Human SARM1 inhibitory state bounded with inhibitor dHNN
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