7z8u
From Proteopedia
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==Catalytic subunit HisG R56A mutant from Psychrobacter arcticus ATPPRT (HisGZ) in complex with ATP and PRPP== | ==Catalytic subunit HisG R56A mutant from Psychrobacter arcticus ATPPRT (HisGZ) in complex with ATP and PRPP== | ||
| - | <StructureSection load='7z8u' size='340' side='right'caption='[[7z8u]]' scene=''> | + | <StructureSection load='7z8u' size='340' side='right'caption='[[7z8u]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z8U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7z8u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psychrobacter_arcticus Psychrobacter arcticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z8U FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z8u OCA], [https://pdbe.org/7z8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z8u RCSB], [https://www.ebi.ac.uk/pdbsum/7z8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z8u ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z8u OCA], [https://pdbe.org/7z8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z8u RCSB], [https://www.ebi.ac.uk/pdbsum/7z8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z8u ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HIS1_PSYA2 HIS1_PSYA2] Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | ATP phosphoribosyltransferase catalyses the first step of histidine biosynthesis and is controlled via a complex allosteric mechanism where the regulatory protein HisZ enhances catalysis by the catalytic protein HisG(S) while mediating allosteric inhibition by histidine. Activation by HisZ was proposed to position HisG(S) Arg56 to stabilise departure of the pyrophosphate leaving group. Here we report active-site mutants of HisG(S) with impaired reaction chemistry which can be allosterically restored by HisZ despite the HisZ:HisG(S) interface lying ~20 A away from the active site. MD simulations indicate HisZ binding constrains the dynamics of HisG(S) to favour a preorganised active site where both Arg56 and Arg32 are poised to stabilise leaving-group departure in WT-HisG(S). In the Arg56Ala-HisG(S) mutant, HisZ modulates Arg32 dynamics so that it can partially compensate for the absence of Arg56. These results illustrate how remote protein-protein interactions translate into catalytic resilience by restoring damaged electrostatic preorganisation at the active site. | ||
| + | |||
| + | Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation.,Fisher G, Corbella M, Alphey MS, Nicholson J, Read BJ, Kamerlin SCL, da Silva RG Nat Commun. 2022 Dec 9;13(1):7607. doi: 10.1038/s41467-022-34960-9. PMID:36494361<ref>PMID:36494361</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7z8u" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[ATP phosphoribosyl transferase 3D structures|ATP phosphoribosyl transferase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Psychrobacter arcticus]] | ||
[[Category: Alphey MS]] | [[Category: Alphey MS]] | ||
[[Category: Fisher G]] | [[Category: Fisher G]] | ||
[[Category: Da Silva RG]] | [[Category: Da Silva RG]] | ||
Revision as of 06:39, 7 April 2023
Catalytic subunit HisG R56A mutant from Psychrobacter arcticus ATPPRT (HisGZ) in complex with ATP and PRPP
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