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| | <StructureSection load='4w78' size='340' side='right'caption='[[4w78]], [[Resolution|resolution]] 1.54Å' scene=''> | | <StructureSection load='4w78' size='340' side='right'caption='[[4w78]], [[Resolution|resolution]] 1.54Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4w78]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctd Myctd] and [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_sumu008 Mycobacterium tuberculosis sumu008]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4W78 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4w78]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_CCDC5180 Mycobacterium tuberculosis CCDC5180] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_SUMu008 Mycobacterium tuberculosis SUMu008]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W78 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4w7b|4w7b]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w78 OCA], [https://pdbe.org/4w78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w78 RCSB], [https://www.ebi.ac.uk/pdbsum/4w78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w78 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CFBR_3763 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=443150 MYCTD]), TMHG_02600 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=675519 Mycobacterium tuberculosis SUMu008])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w78 OCA], [http://pdbe.org/4w78 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w78 RCSB], [http://www.ebi.ac.uk/pdbsum/4w78 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w78 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mycobacterium tuberculosis sumu008]] | + | [[Category: Mycobacterium tuberculosis CCDC5180]] |
| - | [[Category: Myctd]] | + | [[Category: Mycobacterium tuberculosis SUMu008]] |
| - | [[Category: Garcia-Diaz, M]] | + | [[Category: Garcia-Diaz M]] |
| - | [[Category: Guja, K E]] | + | [[Category: Guja KE]] |
| - | [[Category: Sampson, N]] | + | [[Category: Sampson N]] |
| - | [[Category: Yang, M]] | + | [[Category: Yang M]] |
| - | [[Category: Hydratase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The Mycobacterium tuberculosis (Mtb) igr operon plays an essential role in Mtb cholesterol metabolism, which is critical for pathogenesis during the latent stage of Mtb infection. Here we report the first structure of a heterotetrameric MaoC-like enoyl-CoA hydratase, ChsH1-ChsH2, which is encoded by two adjacent genes from the igr operon. We demonstrate that ChsH1-ChsH2 catalyzes the hydration of a steroid enoyl-CoA, 3-oxo-4,17-pregnadiene-20-carboxyl-CoA, in the modified beta-oxidation pathway for cholesterol side chain degradation. The ligand-bound and apoenzyme structures of ChsH1-ChsH2N reveal an unusual, modified hot-dog fold with a severely truncated central alpha-helix that creates an expanded binding site to accommodate the bulkier steroid ring system. The structures show quaternary structure shifts that accommodate the four rings of the steroid substrate and offer an explanation for why the unusual heterotetrameric assembly is utilized for hydration of this steroid. The unique alphabeta heterodimer architecture utilized by ChsH1-ChsH2 to bind its distinctive substrate highlights an opportunity for the development of new antimycobacterial drugs that target a pathway specific to Mtb.
A Distinct MaoC-like Enoyl-CoA Hydratase Architecture Mediates Cholesterol Catabolism in Mycobacterium tuberculosis.,Yang M, Guja KE, Thomas ST, Garcia-Diaz M, Sampson NS ACS Chem Biol. 2014 Sep 22. PMID:25203216[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yang M, Guja KE, Thomas ST, Garcia-Diaz M, Sampson NS. A Distinct MaoC-like Enoyl-CoA Hydratase Architecture Mediates Cholesterol Catabolism in Mycobacterium tuberculosis. ACS Chem Biol. 2014 Sep 22. PMID:25203216 doi:http://dx.doi.org/10.1021/cb500232h
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