1kft
From Proteopedia
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'''Solution Structure of the C-Terminal domain of UvrC from E-coli''' | '''Solution Structure of the C-Terminal domain of UvrC from E-coli''' | ||
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[[Category: Singh, S.]] | [[Category: Singh, S.]] | ||
[[Category: Wechselberger, R.]] | [[Category: Wechselberger, R.]] | ||
| - | [[Category: | + | [[Category: Dna-binding domain]] |
| - | [[Category: | + | [[Category: Helix-hairpin-helix]] |
| - | [[Category: | + | [[Category: Hhh domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:41:40 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:41, 2 May 2008
Solution Structure of the C-Terminal domain of UvrC from E-coli
Overview
The C-terminal domain of the UvrC protein (UvrC CTD) is essential for 5' incision in the prokaryotic nucleotide excision repair process. We have determined the three-dimensional structure of the UvrC CTD using heteronuclear NMR techniques. The structure shows two helix-hairpin-helix (HhH) motifs connected by a small connector helix. The UvrC CTD is shown to mediate structure-specific DNA binding. The domain binds to a single-stranded-double-stranded junction DNA, with a strong specificity towards looped duplex DNA that contains at least six unpaired bases per loop ("bubble DNA"). Using chemical shift perturbation experiments, the DNA-binding surface is mapped to the first hairpin region encompassing the conserved glycine-valine-glycine residues followed by lysine-arginine-arginine, a positively charged surface patch and the second hairpin region consisting of glycine-isoleucine-serine. A model for the protein-DNA complex is proposed that accounts for this specificity.
About this Structure
1KFT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli., Singh S, Folkers GE, Bonvin AM, Boelens R, Wechselberger R, Niztayev A, Kaptein R, EMBO J. 2002 Nov 15;21(22):6257-66. PMID:12426397 Page seeded by OCA on Fri May 2 22:41:40 2008
