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| | <StructureSection load='4wd3' size='340' side='right'caption='[[4wd3]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='4wd3' size='340' side='right'caption='[[4wd3]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wd3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WD3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WD3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wd3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WD3 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vmm|3vmm]], [[3vot|3vot]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wd3 OCA], [https://pdbe.org/4wd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wd3 RCSB], [https://www.ebi.ac.uk/pdbsum/4wd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wd3 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rizA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=135461 Bacillus subtilis subsp. subtilis])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wd3 OCA], [http://pdbe.org/4wd3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wd3 RCSB], [http://www.ebi.ac.uk/pdbsum/4wd3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wd3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/RIZA_BACIU RIZA_BACIU] Catalyzes the synthesis of Arg-Xaa dipeptides in an ATP-dependent manner. Has strict specificity toward arginine as the N-terminal substrate.<ref>PMID:19352016</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Bacillus subtilis subsp. subtilis]] | | [[Category: Bacillus subtilis subsp. subtilis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Arai, T]] | + | [[Category: Arai T]] |
| - | [[Category: Kagawa, W]] | + | [[Category: Kagawa W]] |
| - | [[Category: Kino, K]] | + | [[Category: Kino K]] |
| - | [[Category: Kurumizaka, H]] | + | [[Category: Kurumizaka H]] |
| - | [[Category: Atp-grasp domain]]
| + | |
| - | [[Category: L-amino acid ligase]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Rhizocticin antibiotic biosynthesis]]
| + | |
| Structural highlights
Function
RIZA_BACIU Catalyzes the synthesis of Arg-Xaa dipeptides in an ATP-dependent manner. Has strict specificity toward arginine as the N-terminal substrate.[1]
Publication Abstract from PubMed
RizA is an L-amino-acid ligase from Bacillus subtilis that participates in the biosynthesis of rhizocticin, an oligopeptide antibiotic. The substrate-free form of RizA has been crystallized and the structure was solved at 2.8 A resolution. The amino-acid-binding site appears to be capable of accommodating multiple amino acids, consistent with previous biochemical studies.
Structure of RizA, an L-amino-acid ligase from Bacillus subtilis.,Kagawa W, Arai T, Ishikura S, Kino K, Kurumizaka H Acta Crystallogr F Struct Biol Commun. 2015 Sep;71(Pt 9):1125-30. doi:, 10.1107/S2053230X15012698. Epub 2015 Aug 25. PMID:26323296[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kino K, Kotanaka Y, Arai T, Yagasaki M. A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin. Biosci Biotechnol Biochem. 2009 Apr 23;73(4):901-7. PMID:19352016 doi:10.1271/bbb.80842
- ↑ Kagawa W, Arai T, Ishikura S, Kino K, Kurumizaka H. Structure of RizA, an L-amino-acid ligase from Bacillus subtilis. Acta Crystallogr F Struct Biol Commun. 2015 Sep;71(Pt 9):1125-30. doi:, 10.1107/S2053230X15012698. Epub 2015 Aug 25. PMID:26323296 doi:http://dx.doi.org/10.1107/S2053230X15012698
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