|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4weu' size='340' side='right'caption='[[4weu]], [[Resolution|resolution]] 2.61Å' scene=''> | | <StructureSection load='4weu' size='340' side='right'caption='[[4weu]], [[Resolution|resolution]] 2.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4weu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WEU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4weu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WEU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hlr|3hlr]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4weu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4weu OCA], [https://pdbe.org/4weu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4weu RCSB], [https://www.ebi.ac.uk/pdbsum/4weu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4weu ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">faeG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4weu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4weu OCA], [http://pdbe.org/4weu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4weu RCSB], [http://www.ebi.ac.uk/pdbsum/4weu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4weu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FAEG3_ECOLX FAEG3_ECOLX]] K88 major fimbrial subunit. Fimbriae (also called pili), are polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell. They enable bacteria to colonize the epithelium of specific host organs. | + | [https://www.uniprot.org/uniprot/R9W3F6_LAMGL R9W3F6_LAMGL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 20: |
| | ==See Also== | | ==See Also== |
| | *[[Antibody 3D structures|Antibody 3D structures]] | | *[[Antibody 3D structures|Antibody 3D structures]] |
| - | *[[Pilin|Pilin]] | + | *[[Pilin 3D structures|Pilin 3D structures]] |
| | + | *[[3D structures of non-human antibody|3D structures of non-human antibody]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Camelus glama]] | + | [[Category: Lama glama]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Broeck, I Van den]] | + | [[Category: De Greve H]] |
| - | [[Category: Greve, H De]]
| + | [[Category: De Kerpel M]] |
| - | [[Category: Kerpel, M De]] | + | [[Category: Moonens K]] |
| - | [[Category: Moonens, K]] | + | [[Category: Pardon E]] |
| - | [[Category: Pardon, E]] | + | [[Category: Remaut H]] |
| - | [[Category: Remaut, H]] | + | [[Category: Van den Broeck I]] |
| - | [[Category: Adhesin]] | + | |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Llama single domain antibody]]
| + | |
| - | [[Category: Nanobody]]
| + | |
| Structural highlights
Function
R9W3F6_LAMGL
Publication Abstract from PubMed
Enterotoxigenic Escherichia coli that cause neonatal and post-weaning diarrhea in piglets express F4 fimbriae to mediate attachment towards host receptors. Recently we described how llama single domain antibodies (VHHs) fused to IgA, produced in Arabidopsis thaliana seeds and fed to piglets resulted in a progressive decline in shedding of F4 positive ETEC bacteria. Here we present the structures of these inhibiting VHHs in complex with the major adhesive subunit FaeG. A conserved surface, distant from the lactose binding pocket, is targeted by these VHHs, highlighting the possibility of targeting epitopes on single-domain adhesins that are non-involved in receptor binding.
Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies.,Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H Vet Res. 2015 Feb 24;46(1):14. doi: 10.1186/s13567-015-0151-x. PMID:25828907[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H. Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies. Vet Res. 2015 Feb 24;46(1):14. doi: 10.1186/s13567-015-0151-x. PMID:25828907 doi:http://dx.doi.org/10.1186/s13567-015-0151-x
|
