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| | <StructureSection load='4wl1' size='340' side='right'caption='[[4wl1]], [[Resolution|resolution]] 5.99Å' scene=''> | | <StructureSection load='4wl1' size='340' side='right'caption='[[4wl1]], [[Resolution|resolution]] 5.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wl1]] is a 32 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WL1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WL1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wl1]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WL1 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wl1 OCA], [http://pdbe.org/4wl1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wl1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wl1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wl1 ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wl1 OCA], [https://pdbe.org/4wl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wl1 RCSB], [https://www.ebi.ac.uk/pdbsum/4wl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wl1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/WZZE_ECO57 WZZE_ECO57] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Escherichia coli O157:H7]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cherney, M]] | + | [[Category: Cherney M]] |
| - | [[Category: Cygler, M]] | + | [[Category: Cygler M]] |
| - | [[Category: Kalynych, S]] | + | [[Category: Kalynych S]] |
| - | [[Category: Chain length regulator]]
| + | |
| - | [[Category: Eca]]
| + | |
| - | [[Category: Enterobacterial common antigen]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Polysaccharide co-polymerase]]
| + | |
| Structural highlights
Function
WZZE_ECO57
Publication Abstract from PubMed
Bacteria have evolved cellular control mechanisms to ensure proper length specification for surface-bound polysaccharides. Members of the Polysaccharide Co-Polymerase (PCP) family are central to this process. PCP-1 family members are anchored to the inner membrane through two transmembrane helices and contain a large periplasm-exposed domain. PCPs are known to form homooligomers but their exact stoichiometry is controversial in view of conflicting structural and biochemical data. Several prior investigations addressing this question indicated a nonameric, hexameric or tetrameric organization of several PCP-1 family members. In this work, we gathered additional evidence that E.coli WzzB and WzzE PCPs form octameric homo-oligomeric complexes. Detergent-solubilized PCPs were purified to homogeneity and subjected to blue native gel analysis, which indicated the presence of a predominant high-molecular product of over 500 kDa in mass. Molecular mass of WzzE and WzzB-detergent oligomers was estimated to be 550 kDA by size-exclusion coupled to multi-angle laser light scattering (SEC-MALLS). Oligomeric organization of purified WzzB and WzzE was further investigated by negative stain electron microscopy and by X-ray crystallography, respectively. Analysis of EM-derived molecular envelope of WzzB indicated that the full-length protein is composed of eight protomers. Crystal structure of LDAO-solubilized WzzE was solved to 6A resolution and revealed its octameric subunit stoichiometry. In summary, we identified a possible biological unit utilized for the glycan chain length determination by two PCP-1 family members. This provides an important step toward further unraveling of the mechanistic basis of chain length control of the O-antigen and the enterobacterial common antigen.
Quaternary structure of WzzB and WzzE polysaccharide co-polymerases.,Kalynych S, Cherney M, Bostina M, Rouiller I, Cygler M Protein Sci. 2014 Oct 11. doi: 10.1002/pro.2586. PMID:25307743[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kalynych S, Cherney M, Bostina M, Rouiller I, Cygler M. Quaternary structure of WzzB and WzzE polysaccharide co-polymerases. Protein Sci. 2014 Oct 11. doi: 10.1002/pro.2586. PMID:25307743 doi:http://dx.doi.org/10.1002/pro.2586
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