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| | <StructureSection load='4wu0' size='340' side='right'caption='[[4wu0]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='4wu0' size='340' side='right'caption='[[4wu0]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wu0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cloab Cloab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WU0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wu0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_acetobutylicum_ATCC_824 Clostridium acetobutylicum ATCC 824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WU0 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CA_C0359 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272562 CLOAB])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wu0 OCA], [https://pdbe.org/4wu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wu0 RCSB], [https://www.ebi.ac.uk/pdbsum/4wu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wu0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wu0 OCA], [http://pdbe.org/4wu0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4wu0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wu0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q97M41_CLOAB Q97M41_CLOAB] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cloab]] | + | [[Category: Clostridium acetobutylicum ATCC 824]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gerlach, E S]] | + | [[Category: Gerlach ES]] |
| - | [[Category: Germane, K L]] | + | [[Category: Germane KL]] |
| - | [[Category: Hurley, M M]] | + | [[Category: Hurley MM]] |
| - | [[Category: Servinsky, M D]] | + | [[Category: Servinsky MD]] |
| - | [[Category: Sund, C J]] | + | [[Category: Sund CJ]] |
| - | [[Category: Glycoside hydrolase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Pectin]]
| + | |
| - | [[Category: Rhamnogalacturonan]]
| + | |
| - | [[Category: Unsaturated rhamnogalacturonan hydrolase]]
| + | |
| - | [[Category: Yter]]
| + | |
| Structural highlights
Function
Q97M41_CLOAB
Publication Abstract from PubMed
Clostridium acetobutylicum ATCC 824 gene CA_C0359 encodes a putative unsaturated rhamnogalacturonyl hydrolase (URH) with distant amino-acid sequence homology to YteR of Bacillus subtilis strain 168. YteR, like other URHs, has core structural homology to unsaturated glucuronyl hydrolases, but hydrolyzes the unsaturated disaccharide derivative of rhamnogalacturonan I. The crystal structure of the recombinant CA_C0359 protein was solved to 1.6 A resolution by molecular replacement using the phase information of the previously reported structure of YteR (PDB entry 1nc5) from Bacillus subtilis strain 168. The YteR-like protein is a six-alpha-hairpin barrel with two beta-sheet strands and a small helix overlaying the end of the hairpins next to the active site. The protein has low primary protein sequence identity to YteR but is structurally similar. The two tertiary structures align with a root-mean-square deviation of 1.4 A and contain a highly conserved active pocket. There is a conserved aspartic acid residue in both structures, which has been shown to be important for hydration of the C=C bond during the release of unsaturated galacturonic acid by YteR. A surface electrostatic potential comparison of CA_C0359 and proteins from CAZy families GH88 and GH105 reveals the make-up of the active site to be a combination of the unsaturated rhamnogalacturonyl hydrolase and the unsaturated glucuronyl hydrolase from Bacillus subtilis strain 168. Structural and electrostatic comparisons suggests that the protein may have a slightly different substrate specificity from that of YteR.
Structural analysis of Clostridium acetobutylicum ATCC 824 glycoside hydrolase from CAZy family GH105.,Germane KL, Servinsky MD, Gerlach ES, Sund CJ, Hurley MM Acta Crystallogr F Struct Biol Commun. 2015 Aug 1;71(Pt 8):1100-8. doi:, 10.1107/S2053230X15012121. Epub 2015 Jul 29. PMID:26249707[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Germane KL, Servinsky MD, Gerlach ES, Sund CJ, Hurley MM. Structural analysis of Clostridium acetobutylicum ATCC 824 glycoside hydrolase from CAZy family GH105. Acta Crystallogr F Struct Biol Commun. 2015 Aug 1;71(Pt 8):1100-8. doi:, 10.1107/S2053230X15012121. Epub 2015 Jul 29. PMID:26249707 doi:http://dx.doi.org/10.1107/S2053230X15012121
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