1khr

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[[Image:1khr.gif|left|200px]]
[[Image:1khr.gif|left|200px]]
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{{Structure
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|PDB= 1khr |SIZE=350|CAPTION= <scene name='initialview01'>1khr</scene>, resolution 2.8&Aring;
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The line below this paragraph, containing "STRUCTURE_1khr", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=VIR:VIRGINIAMYCIN+M1'>VIR</scene>
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|GENE= satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 Enterococcus faecium])
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{{STRUCTURE_1khr| PDB=1khr | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1khr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khr OCA], [http://www.ebi.ac.uk/pdbsum/1khr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1khr RCSB]</span>
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'''Crystal Structure of Vat(D) in Complex with Virginiamycin and Coenzyme A'''
'''Crystal Structure of Vat(D) in Complex with Virginiamycin and Coenzyme A'''
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[[Category: Roderick, S L.]]
[[Category: Roderick, S L.]]
[[Category: Sugantino, M.]]
[[Category: Sugantino, M.]]
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[[Category: acyltransferase]]
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[[Category: Acyltransferase]]
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[[Category: antibiotic resistance]]
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[[Category: Antibiotic resistance]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:45:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:48:46 2008''
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Revision as of 19:45, 2 May 2008

Image:1khr.gif

Template:STRUCTURE 1khr

Crystal Structure of Vat(D) in Complex with Virginiamycin and Coenzyme A


Overview

The streptogramin class of antibiotics act to inhibit bacterial protein synthesis, and their semisynthetic derivatives, such as dalfopristin-quinupristin (Synercid), are used to treat serious or life-threatening infections due to multiply antibiotic resistant bacteria. Acquired resistance of the nosocomial pathogen Enterococcus faecium to the group A component of natural and semisynthetic streptogramin mixtures is a prerequisite for the streptogramin resistance phenotype and is mediated by a streptogramin acetyltransferase. The crystal structure of Vat(D), a streptogramin acetyltransferase from a human urinary isolate of E. faecium, has been determined as an apoenzyme and in complex with either acetyl-CoA or virginiamycin M1 and CoA. These structures illustrate the location and arrangement of residues at the active site, and point to His 82 as a residue that may function as a general base. The structural similarity of Vat(D) to the xenobiotic acetyltransferase from Pseudomonas aeruginosa indicates similarities in the catalytic mechanism for these enzymes as well as several shared and distinctive antibiotic binding interactions between these enzymes and their respective substrates. These results reveal the molecular basis for a reaction by which Gram-positive cocci acquire resistance to a last resort antibiotic.

About this Structure

1KHR is a Single protein structure of sequence from Enterococcus faecium. Full crystallographic information is available from OCA.

Reference

Crystal structure of Vat(D): an acetyltransferase that inactivates streptogramin group A antibiotics., Sugantino M, Roderick SL, Biochemistry. 2002 Feb 19;41(7):2209-16. PMID:11841212 Page seeded by OCA on Fri May 2 22:45:50 2008

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