8ed4
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the complex between the arsenite oxidase and its native electron acceptor cytochrome c552 from Pseudorhizobium sp. str. NT-26== | |
| + | <StructureSection load='8ed4' size='340' side='right'caption='[[8ed4]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ed4]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudorhizobium_banfieldiae Pseudorhizobium banfieldiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ED4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ED4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ed4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ed4 OCA], [https://pdbe.org/8ed4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ed4 RCSB], [https://www.ebi.ac.uk/pdbsum/8ed4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ed4 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q6VAL8_9HYPH Q6VAL8_9HYPH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The arsenite oxidase (AioAB) from Pseudorhizobium banfieldiae sp. strain NT-26 catalyzes the oxidation of arsenite to arsenate and transfers electrons to its cognate electron acceptor cytochrome c(552) (cytc(552)). This activity underpins the ability of this organism to respire using arsenite present in contaminated environments. The crystal structure of the AioAB/cytc(552) electron transfer complex reveals two A(2)B(2)/(cytc(552))(2) assemblies per asymmetric unit. Three of the four cytc(552) molecules in the asymmetric unit dock to AioAB in a cleft at the interface between the AioA and AioB subunits, with an edge-to-edge distance of 7.5 A between the heme of cytc(552) and the [2Fe-2S] Rieske cluster in the AioB subunit. The interface between the AioAB and cytc(552) proteins features electrostatic and nonpolar interactions and is stabilized by two salt bridges. A modest number of hydrogen bonds, salt bridges and relatively small, buried surface areas between protein partners are typical features of transient electron transfer complexes. Interestingly, the fourth cytc(552) molecule is positioned differently between two AioAB heterodimers, with distances between its heme and the AioAB redox active cofactors that are outside the acceptable range for fast electron transfer. This unique cytc(552) molecule appears to be positioned to facilitate crystal packing rather than reflecting a functional complex. | ||
| - | + | The structure of the complex between the arsenite oxidase from Pseudorhizobium banfieldiae sp. strain NT-26 and its native electron acceptor cytochrome c(552).,Poddar N, Santini JM, Maher MJ Acta Crystallogr D Struct Biol. 2023 Apr 1;79(Pt 4):345-352. doi: , 10.1107/S2059798323002103. Epub 2023 Mar 30. PMID:36995233<ref>PMID:36995233</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8ed4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudorhizobium banfieldiae]] | ||
| + | [[Category: Maher MJ]] | ||
| + | [[Category: Poddar N]] | ||
Revision as of 20:32, 12 April 2023
Structure of the complex between the arsenite oxidase and its native electron acceptor cytochrome c552 from Pseudorhizobium sp. str. NT-26
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